2fo8: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| (8 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
== | ==Solution structure of the Trypanosoma cruzi cysteine protease inhibitor chagasin== | ||
[[2fo8]] is a 1 chain structure with sequence from [ | <StructureSection load='2fo8' size='340' side='right'caption='[[2fo8]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2fo8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FO8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fo8 OCA], [https://pdbe.org/2fo8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fo8 RCSB], [https://www.ebi.ac.uk/pdbsum/2fo8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fo8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CHAG_TRYCR CHAG_TRYCR] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/2fo8_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fo8 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A Trypanosoma cruzi cysteine protease inhibitor, termed chagasin, is the first characterized member of a new family of tight-binding cysteine protease inhibitors identified in several lower eukaryotes and prokaryotes but not present in mammals. In the protozoan parasite T.cruzi, chagasin plays a role in parasite differentiation and in mammalian host cell invasion, due to its ability to modulate the endogenous activity of cruzipain, a lysosomal-like cysteine protease. In the present work, we determined the solution structure of chagasin and studied its backbone dynamics by NMR techniques. Structured as a single immunoglobulin-like domain in solution, chagasin exerts its inhibitory activity on cruzipain through conserved residues placed in three loops in the same side of the structure. One of these three loops, L4, predicted to be of variable length among chagasin homologues, is flexible in solution as determined by measurements of (15)N relaxation. The biological implications of structural homology between chagasin and other members of the immunoglobulin super-family are discussed. | |||
Solution structure and backbone dynamics of the Trypanosoma cruzi cysteine protease inhibitor chagasin.,Salmon D, do Aido-Machado R, Diehl A, Leidert M, Schmetzer O, de A Lima AP, Scharfstein J, Oschkinat H, Pires JR J Mol Biol. 2006 Apr 14;357(5):1511-21. Epub 2006 Feb 3. PMID:16490204<ref>PMID:16490204</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2fo8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: | |||
[[Category: Trypanosoma cruzi]] | [[Category: Trypanosoma cruzi]] | ||
[[Category: Oschkinat H]] | |||
[[Category: Pires JR]] | |||
[[Category: Salmon D]] | |||
[[Category: Scharfstein J]] | |||
[[Category: De Lima AAP]] | |||
[[Category: Do Aido-Machado R]] | |||