1uty: Difference between revisions

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-[[Image:1uty.png|left|200px]]
-{{STRUCTURE_1uty|  PDB=1uty  |  SCENE=  }}
+==Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)==
+<StructureSection load='1uty' size='340' side='right'caption='[[1uty]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1uty]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bluetongue_virus_8 Bluetongue virus 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UTY FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uty OCA], [https://pdbe.org/1uty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uty RCSB], [https://www.ebi.ac.uk/pdbsum/1uty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uty ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/VNS2_BTV10 VNS2_BTV10] Single-stranded RNA-binding protein.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ut/1uty_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uty ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bluetongue virus non-structural protein 2 belongs to a class of highly conserved proteins found in orbiviruses of the Reoviridae family. Non-structural protein 2 forms large multimeric complexes and localizes to cytoplasmic inclusions in infected cells. It is able to bind single-stranded RNA non-specifically, and it has been suggested that the protein is involved in the selection and condensation of the Bluetongue virus RNA segments prior to genome encapsidation. We have determined the x-ray structure of the N-terminal domain (sufficient for the RNA binding ability of non-structural protein 2) to 2.4 A resolution using anomalous scattering methods. Crystals of this apparently insoluble domain were obtained by in situ proteolysis of a soluble construct. The asymmetric unit shows two monomers related by non-crystallographic symmetry, with each monomer folded as a beta sandwich with a unique topology. The crystal structure reveals extensive monomer-monomer interactions, which explain the ability of the protein to self-assemble into large homomultimeric complexes. Of the entire surface area of the monomer, one-third is used to create the interfaces of the curved multimeric assembly observed in the x-ray structure. The structure reported here shows how the N-terminal domain would be able to bind single-stranded RNA non-specifically protecting the bound regions in a heterogeneous multimeric but not polymeric complex.
-===CRYSTAL STRUCTURE OF THE RNA BINDING DOMAIN OF BLUETONGUE VIRUS NON-STRUCTURAL PROTEIN 2(NS2)===
+Structure and assembly of the RNA binding domain of bluetongue virus non-structural protein 2.,Butan C, Van Der Zandt H, Tucker PA J Biol Chem. 2004 Sep 3;279(36):37613-21. Epub 2004 May 20. PMID:15155766<ref>PMID:15155766</ref>
-{{ABSTRACT_PUBMED_15155766}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1uty" style="background-color:#fffaf0;"></div>
-[[1uty]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus Bluetongue virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTY OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:015155766</ref><references group="xtra"/>
+</StructureSection>
-[[Category: Bluetongue virus]]
+[[Category: Bluetongue virus 8]]
-[[Category: RNA-directed RNA polymerase]]
+[[Category: Large Structures]]
-[[Category: Butan, C.]]
+[[Category: Butan C]]
-[[Category: Tucker, P.]]
+[[Category: Tucker P]]
-[[Category: Zandt, H Van Der.]]
+[[Category: Van Der zandt H]]
-[[Category: Rna binding protein]]
-[[Category: Viral protein]]