1hi0: Difference between revisions

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-[[Image:1hi0.png|left|200px]]
-{{STRUCTURE_1hi0|  PDB=1hi0  |  SCENE=  }}
+==RNA dependent RNA polymerase from dsRNA bacteriophage phi6 plus initiation complex==
+<StructureSection load='1hi0' size='340' side='right'caption='[[1hi0]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1hi0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HI0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hi0 OCA], [https://pdbe.org/1hi0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hi0 RCSB], [https://www.ebi.ac.uk/pdbsum/1hi0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hi0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RDRP_BPPH6 RDRP_BPPH6]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In most RNA viruses, genome replication and transcription are catalysed by a viral RNA-dependent RNA polymerase. Double-stranded RNA viruses perform these operations in a capsid (the polymerase complex), using an enzyme that can read both single- and double-stranded RNA. Structures have been solved for such viral capsids, but they do not resolve the polymerase subunits in any detail. Here we show that the 2 A resolution X-ray structure of the active polymerase subunit from the double-stranded RNA bacteriophage straight phi6 is highly similar to that of the polymerase of hepatitis C virus, providing an evolutionary link between double-stranded RNA viruses and flaviviruses. By crystal soaking and co-crystallization, we determined a number of other structures, including complexes with oligonucleotide and/or nucleoside triphosphates (NTPs), that suggest a mechanism by which the incoming double-stranded RNA is opened up to feed the template through to the active site, while the substrates enter by another route. The template strand initially overshoots, locking into a specificity pocket, and then, in the presence of cognate NTPs, reverses to form the initiation complex; this process engages two NTPs, one of which acts with the carboxy-terminal domain of the protein to prime the reaction. Our results provide a working model for the initiation of replication and transcription.
-===RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS INITIATION COMPLEX===
+A mechanism for initiating RNA-dependent RNA polymerization.,Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI Nature. 2001 Mar 8;410(6825):235-40. PMID:11242087<ref>PMID:11242087</ref>
-{{ABSTRACT_PUBMED_11242087}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1hi0" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[1hi0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HI0 OCA].
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:011242087</ref><ref group="xtra">PMID:011053857</ref><references group="xtra"/>
+__TOC__
-[[Category: Pseudomonas phage phi6]]
+</StructureSection>
-[[Category: Bamford, D H.]]
+[[Category: Large Structures]]
-[[Category: Butcher, S J.]]
+[[Category: Pseudomonas virus phi6]]
-[[Category: Grimes, J M.]]
+[[Category: Bamford DH]]
-[[Category: Makeyev, E V.]]
+[[Category: Butcher SJ]]
-[[Category: Stuart, D I.]]
+[[Category: Grimes JM]]
-[[Category: Rna polymerase]]
+[[Category: Makeyev EV]]
-[[Category: Viral polymerase]]
+[[Category: Stuart DI]]