1e7k: Difference between revisions

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-[[Image:1e7k.gif|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_1e7k", creates the "Structure Box" on the page.
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-{{STRUCTURE_1e7k|  PDB=1e7k  |  SCENE=  }}
-'''CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT'''
+==Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment==
+<StructureSection load='1e7k' size='340' side='right'caption='[[1e7k]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E7K FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [https://pdbe.org/1e7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [https://www.ebi.ac.uk/pdbsum/1e7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7k ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/1e7k_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e7k ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.
-==Overview==
+Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.,Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R Mol Cell. 2000 Dec;6(6):1331-42. PMID:11163207<ref>PMID:11163207</ref>
-We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-E7K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA].
+</div>
+<div class="pdbe-citations 1e7k" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment., Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R, Mol Cell. 2000 Dec;6(6):1331-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11163207 11163207]
+*[[Kink-turn motif|Kink-turn motif]]
+*[[User:Wayne Decatur/kink-turn motif|User:Wayne Decatur/kink-turn motif]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Ficner, R.]]
+[[Category: Ficner R]]
-[[Category: Harthmuth, K.]]
+[[Category: Harthmuth K]]
-[[Category: Luhrmann, R.]]
+[[Category: Luhrmann R]]
-[[Category: Nottrott, S.]]
+[[Category: Nottrott S]]
-[[Category: Vidovic, I.]]
+[[Category: Vidovic I]]
-[[Category: Rna recognition motif]]
-[[Category: Splicing]]
-[[Category: U4 snrna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:45:46 2008''