7h2c: Difference between revisions

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'''Unreleased structure'''


The entry 7h2c is ON HOLD
==PanDDA analysis group deposition -- Crystal Structure of ZIKV NS2B-NS3 protease in complex with Z1328968520==
 
<StructureSection load='7h2c' size='340' side='right'caption='[[7h2c]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
Authors: Ni, X., Godoy, A.S., Marples, P.G., Fairhead, M., Balcomb, B.H., Tomlinson, C.W.E., Koekemoer, L., Aschenbrenner, J.C., Lithgo, R.M., Thompson, W., Wild, C., Williams, E.P., Winokan, M., Chandran, A.V., Fearon, D., Walsh, M.A., von Delft, F.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[7h2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zika_virus Zika virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7H2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7H2C FirstGlance]. <br>
Description: PanDDA analysis group deposition --Crystal Structure of ZIKV NS2B-NS3 protease in complex with Z1328968520
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.399&#8491;</td></tr>
[[Category: Unreleased Structures]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=J4Q:4-[(2-methylsulfonylimidazol-1-yl)methyl]-1,3-thiazole'>J4Q</scene></td></tr>
[[Category: Balcomb, B.H]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7h2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7h2c OCA], [https://pdbe.org/7h2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7h2c RCSB], [https://www.ebi.ac.uk/pdbsum/7h2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7h2c ProSAT]</span></td></tr>
[[Category: Chandran, A.V]]
</table>
[[Category: Williams, E.P]]
== Function ==
[[Category: Lithgo, R.M]]
[https://www.uniprot.org/uniprot/POLG_ZIKV POLG_ZIKV] Protein C: Encapsulates the genomic RNA.[UniProtKB:P17763]  prM: Acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated.[UniProtKB:P17763]  Envelope protein E: Binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes.[UniProtKB:P17763]  Non-structural protein 1: Involved in virus replication and regulation of the innate immune response.[UniProtKB:P17763]  Non-structural protein 2A: May be involved viral RNA replication and capsid assembly.[UniProtKB:P09732]  Non-structural protein 4A: Induces host endoplasmic reticulum membrane rearrangements leading to the formation of virus-induced membranous vesicles hosting the dsRNA and polymerase, functioning as a replication complex. NS4A might also regulate the ATPase activity of the helicase region of Serine protease NS3 chain.[UniProtKB:P17763]  Peptide 2k: Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[UniProtKB:P17763]  Non-structural protein 4B: Inhibits interferon (IFN)-induced host STAT1 phosphorylation and nuclear translocation, thereby preventing the establishment of cellular antiviral state by blocking the IFN-alpha/beta pathway.[UniProtKB:P17763]
[[Category: Godoy, A.S]]
__TOC__
[[Category: Fearon, D]]
</StructureSection>
[[Category: Walsh, M.A]]
[[Category: Large Structures]]
[[Category: Thompson, W]]
[[Category: Zika virus]]
[[Category: Fairhead, M]]
[[Category: Aschenbrenner JC]]
[[Category: Von Delft, F]]
[[Category: Balcomb BH]]
[[Category: Marples, P.G]]
[[Category: Chandran AV]]
[[Category: Koekemoer, L]]
[[Category: Fairhead M]]
[[Category: Wild, C]]
[[Category: Fearon D]]
[[Category: Ni, X]]
[[Category: Godoy AS]]
[[Category: Aschenbrenner, J.C]]
[[Category: Koekemoer L]]
[[Category: Winokan, M]]
[[Category: Lithgo RM]]
[[Category: Tomlinson, C.W.E]]
[[Category: Marples PG]]
[[Category: Ni X]]
[[Category: Thompson W]]
[[Category: Tomlinson CWE]]
[[Category: Walsh MA]]
[[Category: Wild C]]
[[Category: Williams EP]]
[[Category: Winokan M]]
[[Category: Von Delft F]]

Latest revision as of 11:26, 9 May 2024

PanDDA analysis group deposition -- Crystal Structure of ZIKV NS2B-NS3 protease in complex with Z1328968520PanDDA analysis group deposition -- Crystal Structure of ZIKV NS2B-NS3 protease in complex with Z1328968520

Structural highlights

7h2c is a 2 chain structure with sequence from Zika virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.399Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

POLG_ZIKV Protein C: Encapsulates the genomic RNA.[UniProtKB:P17763] prM: Acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated.[UniProtKB:P17763] Envelope protein E: Binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes.[UniProtKB:P17763] Non-structural protein 1: Involved in virus replication and regulation of the innate immune response.[UniProtKB:P17763] Non-structural protein 2A: May be involved viral RNA replication and capsid assembly.[UniProtKB:P09732] Non-structural protein 4A: Induces host endoplasmic reticulum membrane rearrangements leading to the formation of virus-induced membranous vesicles hosting the dsRNA and polymerase, functioning as a replication complex. NS4A might also regulate the ATPase activity of the helicase region of Serine protease NS3 chain.[UniProtKB:P17763] Peptide 2k: Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[UniProtKB:P17763] Non-structural protein 4B: Inhibits interferon (IFN)-induced host STAT1 phosphorylation and nuclear translocation, thereby preventing the establishment of cellular antiviral state by blocking the IFN-alpha/beta pathway.[UniProtKB:P17763]

7h2c, resolution 1.40Å

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