1tz4: Difference between revisions

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-[[Image:1tz4.gif|left|200px]]<br /><applet load="1tz4" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1tz4" />
-'''[hPP19-23]-pNPY bound to DPC Micelles'''<br />
-==Overview==
+==[hPP19-23]-pNPY bound to DPC Micelles==
-Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NPY segment 19-23 into PP leads to an increase in affinity at the Y(1) and Y(2) receptor subtypes whereas the exchange of this segment from PP into NPY leads to a large decrease in affinity at all receptor subtypes. PP displays a very stable structure in solution, with the N terminus being back-folded onto the C-terminal alpha-helix (the so-called PP-fold). The helix of NPY is less stable and the N terminus is freely diffusing in solution. The exchange of this segment, however, does not alter the PP-fold propensities of the chimeric peptides in solution. The structures of the phospholipid micelle-bound peptides serving to mimic the membrane-bound species display segregation into a more flexible N-terminal region and a well-defined alpha-helical region. The introduction of the [19-23]-pNPY segment into hPP leads to an N-terminal extension of the alpha-helix, now starting at Pro(14) instead of Met(17). In contrast, a truncated helix is observed in [(19)(-)(23)hPP]-pNPY, starting at Leu(17) instead of Ala(14). All peptides display moderate binding affinities to neutral membranes (K(assoc) in the range of 1.7 to 6.8 x 10(4) mol(-)(1) as determined by surface plasmon resonance) with the differences in binding being most probably related to the exchange of Arg-19 (pNPY) by Glu-23 (hPP). Differences in receptor binding properties between the chimeras and their parental peptides are therefore most likely due to changes in the conformation of the micelle-bound peptides.
+<StructureSection load='1tz4' size='340' side='right'caption='[[1tz4]]' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1tz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TZ4 FirstGlance]. <br>
-TZ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa_and_homo_sapiens Sus scrofa and homo sapiens] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZ4 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tz4 OCA], [https://pdbe.org/1tz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tz4 RCSB], [https://www.ebi.ac.uk/pdbsum/1tz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tz4 ProSAT]</span></td></tr>
-Strongly altered receptor binding properties in PP and NPY chimeras are accompanied by changes in structure and membrane binding., Lerch M, Kamimori H, Folkers G, Aguilar MI, Beck-Sickinger AG, Zerbe O, Biochemistry. 2005 Jun 28;44(25):9255-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15966750 15966750]
+</table>
-[[Category: Single protein]]
+== Function ==
-[[Category: Sus scrofa and homo sapiens]]
+[https://www.uniprot.org/uniprot/NPY_PIG NPY_PIG] NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.[https://www.uniprot.org/uniprot/PAHO_HUMAN PAHO_HUMAN] Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.  The physiological role for the icosapeptide has not yet been elucidated.
-[[Category: Aguilar, M I.]]
+== Evolutionary Conservation ==
-[[Category: Beck-Sickinger, A G.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Folkers, G.]]
+Check<jmol>
-[[Category: Kamimori, H.]]
+  <jmolCheckbox>
-[[Category: Lerch, M.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tz/1tz4_consurf.spt"</scriptWhenChecked>
-[[Category: Zerbe, O.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: NH2]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: npy-pp chimera]]
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tz4 ConSurf].
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:53 2008''
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Sus scrofa]]
+[[Category: Aguilar MI]]
+[[Category: Beck-Sickinger AG]]
+[[Category: Folkers G]]
+[[Category: Kamimori H]]
+[[Category: Lerch M]]
+[[Category: Zerbe O]]