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[[Image:1tkq.gif|left|200px]]<br /><applet load="1tkq" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1tkq" />
'''SOLUTION STRUCTURE OF A LINKED UNSYMMETRIC GRAMICIDIN IN A MEMBRANE-ISOELECTRICAL SOLVENTS MIXTURE IN THE PRESENCE OF CsCl'''<br />


==Overview==
==SOLUTION STRUCTURE OF A LINKED UNSYMMETRIC GRAMICIDIN IN A MEMBRANE-ISOELECTRICAL SOLVENTS MIXTURE IN THE PRESENCE OF CsCl==
The biological ion channel gramicidin A (gA) was modified by synthetic means to obtain the tail-to-tail linked asymmetric gA-derived dimer compound 3. Single-channel current measurements for 3 in planar lipid bilayers exhibit an Eisenman I ion selectivity for alkali cations. The structural asymmetry does not lead to an observable functional asymmetry. The structure of 3 in solution without and with Cs cations was investigated by 1H-NMR spectroscopy. In CDCl3/CD3OH (1 : 1, v/v), 3 forms a mixture of double-stranded beta-helices. Upon addition of excess CsCl, the double-stranded species are converted completely into one new conformer: the right-handed single-stranded beta-helix. A combination of DQF-COSY and TOCSY was used for the assignment of the 1H-NMR spectrum of the Cs-3 complex in CDCl3/CD3OH (1 : 1, v/v). A total of 69 backbone, 27 long-range, and 64 side-chain distance restraints were obtained from NOESY together with 25 phi and 14 chi1 torsion angles obtained from coupling constants. These data were used as input for structure calculation with dyana built in sybyl 6.8. A final set of 11 structures with an average rmsd for the backbone of 0.45 A was obtained (PDB: 1TKQ). The structure of the Cs-3 complex in solution is equivalent to the bioactive channel conformation in the membrane environment.
<StructureSection load='1tkq' size='340' side='right'caption='[[1tkq]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1tkq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TKQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DLE:D-LEUCINE'>DLE</scene>, <scene name='pdbligand=DVA:D-VALINE'>DVA</scene>, <scene name='pdbligand=PRD_000155:MINI-GRAMICIDIN+A+-+GRAMICIDIN+A+DIMER'>PRD_000155</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tkq OCA], [https://pdbe.org/1tkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tkq RCSB], [https://www.ebi.ac.uk/pdbsum/1tkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tkq ProSAT]</span></td></tr>
</table>


==About this Structure==
==See Also==
1TKQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKQ OCA].
*[[Gramicidin|Gramicidin]]
 
__TOC__
==Reference==
</StructureSection>
An asymmetric ion channel derived from gramicidin A. Synthesis, function and NMR structure., Xie X, Al-Momani L, Reiss P, Griesinger C, Koert U, FEBS J. 2005 Feb;272(4):975-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15691331 15691331]
[[Category: Brevibacillus brevis]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Al-Momani, L.]]
[[Category: Al-Momani L]]
[[Category: Bockelmann, D.]]
[[Category: Bockelmann D]]
[[Category: Griesinger, C.]]
[[Category: Griesinger C]]
[[Category: Koert, U.]]
[[Category: Koert U]]
[[Category: Xie, X.]]
[[Category: Xie X]]
[[Category: beta-helix]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:14:38 2008''

Latest revision as of 11:42, 1 May 2024

SOLUTION STRUCTURE OF A LINKED UNSYMMETRIC GRAMICIDIN IN A MEMBRANE-ISOELECTRICAL SOLVENTS MIXTURE IN THE PRESENCE OF CsClSOLUTION STRUCTURE OF A LINKED UNSYMMETRIC GRAMICIDIN IN A MEMBRANE-ISOELECTRICAL SOLVENTS MIXTURE IN THE PRESENCE OF CsCl

Structural highlights

1tkq is a 2 chain structure with sequence from Brevibacillus brevis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

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