1tat: Difference between revisions

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New page: left|200px<br /><applet load="1tat" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tat, resolution 3.0Å" /> '''CRYSTALLINE MITOCHOND...
 
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'''CRYSTALLINE MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE EXISTS IN ONLY TWO CONFORMATIONS'''<br />


==Overview==
==CRYSTALLINE MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE EXISTS IN ONLY TWO CONFORMATIONS==
The subunits of the alpha 2-dimeric enzyme aspartate aminotransferase are, composed of two distinct domains, one large and one small. The active, sites are situated close to both the intersubunit and the interdomain, interface. Binding of substrate analogues to the active site induces a, large conformational change in the enzyme, whereby the small domain, rotates by 13 degrees relative to the large domain and completely buries, the ligand. We have determined the crystal structures of chicken, mitochondrial aspartate aminotransferase (mAATase) in two new crystal, forms. A comparison of the structures of mAATase in five crystal forms, including both the unliganded and the liganded enzyme, shows that mAATase, exists in either one of two unique conformations, with only minimal, adaptations to the crystal lattice. This suggests that both the open, unliganded and closed, liganded structure of mAATase are, to a large, extent, stabilized by intramolecular interactions, and are consequently, representative of functional states of the protein in solution. A, 2-fold-symmetric packing interaction between small domains occurring, identically in three crystal forms of mAATase is described.
<StructureSection load='1tat' size='340' side='right'caption='[[1tat]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1tat]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TAT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tat OCA], [https://pdbe.org/1tat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tat RCSB], [https://www.ebi.ac.uk/pdbsum/1tat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tat ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AATM_CHICK AATM_CHICK] Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. May facilitate cellular uptake of long-chain free fatty acids (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ta/1tat_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tat ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1TAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with PLP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TAT OCA].
*[[Aspartate aminotransferase 3D structures|Aspartate aminotransferase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystalline mitochondrial aspartate aminotransferase exists in only two conformations., Hohenester E, Jansonius JN, J Mol Biol. 1994 Mar 4;236(4):963-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8120903 8120903]
[[Category: Aspartate transaminase]]
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Hohenester, E.]]
[[Category: Hohenester E]]
[[Category: Jansonius, J.N.]]
[[Category: Jansonius JN]]
[[Category: PLP]]
[[Category: aminotransferase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:07:30 2007''

Latest revision as of 11:39, 1 May 2024

CRYSTALLINE MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE EXISTS IN ONLY TWO CONFORMATIONSCRYSTALLINE MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE EXISTS IN ONLY TWO CONFORMATIONS

Structural highlights

1tat is a 2 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AATM_CHICK Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. May facilitate cellular uptake of long-chain free fatty acids (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1tat, resolution 3.00Å

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