1t1b: Difference between revisions

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-[[Image:1t1b.gif|left|200px]]
- {{Structure
+==Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP==
-|PDB= 1t1b |SIZE=350|CAPTION= <scene name='initialview01'>1t1b</scene>, resolution 1.60&Aring;
+<StructureSection load='1t1b' size='340' side='right'caption='[[1t1b]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=HC4:4'-HYDROXYCINNAMIC ACID'>HC4</scene>
+<table><tr><td colspan='2'>[[1t1b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1B FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1b OCA], [https://pdbe.org/1t1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1b RCSB], [https://www.ebi.ac.uk/pdbsum/1t1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1b ProSAT]</span></td></tr>
+</table>
-'''Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP'''
+== Function ==
+[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
-In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
+Check<jmol>
+  <jmolCheckbox>
-==About this Structure==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t1b_consurf.spt"</scriptWhenChecked>
-T1B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1B OCA].
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==Reference==
+  </jmolCheckbox>
-A structural pathway for signaling in the E46Q mutant of photoactive yellow protein., Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K, Structure. 2005 Jan;13(1):55-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15642261 15642261]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t1b ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
 [[Category: Halorhodospira halophila]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Anderson, S.]]
+[[Category: Anderson S]]
-[[Category: Moffat, K.]]
+[[Category: Moffat K]]
-[[Category: Pahl, R.]]
+[[Category: Pahl R]]
-[[Category: Rajagopal, S.]]
+[[Category: Rajagopal S]]
-[[Category: Schmidt, M.]]
+[[Category: Schmidt M]]
-[[Category: Srajer, V.]]
+[[Category: Srajer V]]
-[[Category: HC4]]
-[[Category: pas domain]]
-[[Category: photoactive yellow protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:12:23 2008''