1t1a: Difference between revisions

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[[Image:1t1a.gif|left|200px]]


{{Structure
==Late intermediate IL1 from time-resolved crystallography of the E46Q mutant of PYP==
|PDB= 1t1a |SIZE=350|CAPTION= <scene name='initialview01'>1t1a</scene>, resolution 1.60&Aring;
<StructureSection load='1t1a' size='340' side='right'caption='[[1t1a]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=HC4:4&#39;-HYDROXYCINNAMIC+ACID'>HC4</scene>
<table><tr><td colspan='2'>[[1t1a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1A FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1a OCA], [https://pdbe.org/1t1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1a RCSB], [https://www.ebi.ac.uk/pdbsum/1t1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1a ProSAT]</span></td></tr>
|RELATEDENTRY=[[1t18|1T18]], [[1t19|1T19]], [[1t1b|1T1B]], [[1t1c|1T1C]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1a OCA], [http://www.ebi.ac.uk/pdbsum/1t1a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t1a RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
 
== Evolutionary Conservation ==
'''Late intermediate IL1 from time-resolved crystallography of the E46Q mutant of PYP'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t1a_consurf.spt"</scriptWhenChecked>
In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1T1A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1A OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t1a ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
A structural pathway for signaling in the E46Q mutant of photoactive yellow protein., Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K, Structure. 2005 Jan;13(1):55-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15642261 15642261]
</StructureSection>
[[Category: Halorhodospira halophila]]
[[Category: Halorhodospira halophila]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Anderson, S.]]
[[Category: Anderson S]]
[[Category: Moffat, K.]]
[[Category: Moffat K]]
[[Category: Pahl, R.]]
[[Category: Pahl R]]
[[Category: Rajagopal, S.]]
[[Category: Rajagopal S]]
[[Category: Schmidt, M.]]
[[Category: Schmidt M]]
[[Category: Srajer, V.]]
[[Category: Srajer V]]
[[Category: pas domain]]
[[Category: photoactive yellow protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:49:49 2008''

Latest revision as of 11:37, 1 May 2024

Late intermediate IL1 from time-resolved crystallography of the E46Q mutant of PYPLate intermediate IL1 from time-resolved crystallography of the E46Q mutant of PYP

Structural highlights

1t1a is a 1 chain structure with sequence from Halorhodospira halophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PYP_HALHA Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1t1a, resolution 1.60Å

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