1sum: Difference between revisions

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New page: left|200px<br /><applet load="1sum" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sum, resolution 2.00Å" /> '''Crystal structure of...
 
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[[Image:1sum.gif|left|200px]]<br /><applet load="1sum" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1sum, resolution 2.00&Aring;" />
'''Crystal structure of a hypothetical protein at 2.0 A resolution'''<br />


==Overview==
==Crystal structure of a hypothetical protein at 2.0 A resolution==
PhoU proteins are known to play a role in the regulation of phosphate, uptake. In Thermotoga maritima, two PhoU homologues have been identified, bioinformatically. Here we report the crystal structure of one of the PhoU, homologues at 2.0 A resolution. The structure of the PhoU protein, homologue contains a highly symmetric new structural fold composed of two, repeats of a three-helix bundle. The structure unexpectedly revealed a, trinuclear and a tetranuclear iron cluster that were found to be bound on, the surface. Each of the two multinuclear iron clusters is coordinated by, a conserved E(D)XXXD motif pair. Our structure reveals a new class of, metalloprotein containing multinuclear iron clusters. The possible, functional implication based on the structure are discussed.
<StructureSection load='1sum' size='340' side='right'caption='[[1sum]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1sum]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SUM FirstGlance]. <br>
1SUM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with FE, NI, CA and PO3 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SUM OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sum OCA], [https://pdbe.org/1sum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sum RCSB], [https://www.ebi.ac.uk/pdbsum/1sum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sum ProSAT], [https://www.topsan.org/Proteins/BSGC/1sum TOPSAN]</span></td></tr>
Crystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters., Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH, J Biol Chem. 2005 Apr 22;280(16):15960-6. Epub 2005 Feb 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15716271 15716271]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/PHOU2_THEMA PHOU2_THEMA] Plays a role in the regulation of phosphate uptake (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/su/1sum_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sum ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Adams, P.D.]]
[[Category: Adams PD]]
[[Category: BSGC, Berkeley.Structural.Genomics.Center.]]
[[Category: Kim R]]
[[Category: Kim, R.]]
[[Category: Kim SH]]
[[Category: Kim, S.H.]]
[[Category: Liu J]]
[[Category: Liu, J.]]
[[Category: Lou Y]]
[[Category: Lou, Y.]]
[[Category: Yokota H]]
[[Category: Yokota, H.]]
[[Category: CA]]
[[Category: FE]]
[[Category: NI]]
[[Category: PO3]]
[[Category: abc transport]]
[[Category: berkeley structural genomics center]]
[[Category: bsgc]]
[[Category: phou]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: pst]]
[[Category: structural genomics]]
[[Category: structure funded by nih]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:41:04 2007''

Latest revision as of 11:35, 1 May 2024

Crystal structure of a hypothetical protein at 2.0 A resolutionCrystal structure of a hypothetical protein at 2.0 A resolution

Structural highlights

1sum is a 1 chain structure with sequence from Thermotoga maritima. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

PHOU2_THEMA Plays a role in the regulation of phosphate uptake (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1sum, resolution 2.00Å

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