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[[Image:1saz.gif|left|200px]]


{{Structure
==Membership in the ASKHA Superfamily: Enzymological Properties and Crystal Structure of Butyrate Kinase 2 from Thermotoga maritima==
|PDB= 1saz |SIZE=350|CAPTION= <scene name='initialview01'>1saz</scene>, resolution 2.50&Aring;
<StructureSection load='1saz' size='340' side='right'caption='[[1saz]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
<table><tr><td colspan='2'>[[1saz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SAZ FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Butyrate_kinase Butyrate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.7 2.7.2.7] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE= BUK2, TM1756 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1saz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1saz OCA], [https://pdbe.org/1saz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1saz RCSB], [https://www.ebi.ac.uk/pdbsum/1saz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1saz ProSAT]</span></td></tr>
|RELATEDENTRY=[[1g99|1G99]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1saz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1saz OCA], [http://www.ebi.ac.uk/pdbsum/1saz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1saz RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/BUK2_THEMA BUK2_THEMA]
 
== Evolutionary Conservation ==
'''Membership in the ASKHA Superfamily: Enzymological Properties and Crystal Structure of Butyrate Kinase 2 from Thermotoga maritima'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sa/1saz_consurf.spt"</scriptWhenChecked>
The sitting-drop method of crystallization uses the evaporation of water to increase the concentration of the protein and precipitant in the drop. The presence of other volatile components, such as acetic acid, can have a marked impact on crystallization. A member of the ASKHA (acetate and sugar kinases/Hsc70/actin) superfamily of proteins, isobutyrate kinase (Buk2) from Thermotoga maritima, was expressed in Escherichia coli with six histidine residues added to the C-terminus. The purified protein was crystallized in a sitting drop with a well solution consisting of 1.7-3.0 M sodium formate, with the pH of the well solution alone adjusted to 4.5 with acetic acid. Diffraction data collected at 100 K show that the crystals diffract to 3.1 A and belong to space group I422, with unit-cell parameters a = b = 198.12, c = 58.93 A. Both the crystal form and the results of dynamic light-scattering studies suggest that Buk2 is an octomer, the first to be identified in the ASKHA superfamily.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1SAZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAZ OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1saz ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Crystallization of butyrate kinase 2 from Thermotoga maritima mediated by vapor diffusion of acetic acid., Diao J, Cooper DR, Sanders DA, Hasson MS, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1100-2. Epub 2003, May 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12777787 12777787]
</StructureSection>
[[Category: Butyrate kinase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Cooper, D R.]]
[[Category: Cooper DR]]
[[Category: Diao, J S.]]
[[Category: Diao J]]
[[Category: Hasson, M S.]]
[[Category: Hasson MS]]
[[Category: Sanders, D A.]]
[[Category: Sanders DA]]
[[Category: acetate kinase]]
[[Category: actin) superfamily]]
[[Category: amppcp]]
[[Category: askha (acetate and sugar kinase]]
[[Category: butyrate]]
[[Category: butyrate kinase]]
[[Category: disulfide bond]]
[[Category: enzyme mechanism]]
[[Category: hsc70]]
[[Category: isobutyrate]]
[[Category: isobutyrate kinase]]
[[Category: two similar domain]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:37 2008''

Latest revision as of 11:30, 1 May 2024

Membership in the ASKHA Superfamily: Enzymological Properties and Crystal Structure of Butyrate Kinase 2 from Thermotoga maritimaMembership in the ASKHA Superfamily: Enzymological Properties and Crystal Structure of Butyrate Kinase 2 from Thermotoga maritima

Structural highlights

1saz is a 1 chain structure with sequence from Thermotoga maritima. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BUK2_THEMA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1saz, resolution 2.50Å

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OCA
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