1rgj: Difference between revisions

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-{{Seed}}
-[[Image:1rgj.png|left|200px]]
-<!--
+==NMR STRUCTURE OF THE COMPLEX BETWEEN ALPHA-BUNGAROTOXIN AND MIMOTOPE OF THE NICOTINIC ACETYLCHOLINE RECEPTOR WITH ENHANCED ACTIVITY==
-The line below this paragraph, containing "STRUCTURE_1rgj", creates the "Structure Box" on the page.
+<StructureSection load='1rgj' size='340' side='right'caption='[[1rgj]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1rgj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1p67 1p67]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RGJ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rgj OCA], [https://pdbe.org/1rgj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rgj RCSB], [https://www.ebi.ac.uk/pdbsum/1rgj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rgj ProSAT]</span></td></tr>
-{{STRUCTURE_1rgj|  PDB=1rgj  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/3L21A_BUNMU 3L21A_BUNMU] Binds with high affinity to muscular (tested on Torpedo marmorata, Kd=0.4 nM) and neuronal (tested on chimeric alpha-7/CHRNA7, Kd=0.95 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). It also shows an activity on GABA(A) receptors (PubMed:16549768, PubMed:25634239). It antagonises GABA-activated currents with high potency when tested on primary hippocampal neurons (PubMed:25634239). It inhibits recombinantly expressed GABA(A) receptors composed of alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits with high potency (62.3% inhibition at 20 uM of toxin) (PubMed:25634239). It also shows a weaker inhibition on GABA(A) receptors composed of alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) subunits, alpha-4-beta-2-gamma-2 (GABRA4-GABRB2-GABRG2) subunits, and alpha-5-beta-2-gamma-2 (GABRA5-GABRB2-GABRG2) subunits (PubMed:25634239). A very weak inhibition is also observed on GABA(A) receptor composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) (PubMed:26221036). It has also been shown to bind and inhibit recombinant GABA(A) receptor beta-3/GABRB3 subunit (Kd=about 50 nM) (PubMed:16549768). In addition, it blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM) (PubMed:9840221).<ref>PMID:16549768</ref> <ref>PMID:25634239</ref> <ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rg/1rgj_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rgj ConSurf].
+<div style="clear:both"></div>
-===NMR STRUCTURE OF THE COMPLEX BETWEEN ALPHA-BUNGAROTOXIN AND MIMOTOPE OF THE NICOTINIC ACETILCHOLINE RECEPTOR WITH ENHANCED ACTIVITY===
+==See Also==
+*[[Bungarotoxin 3D structures|Bungarotoxin 3D structures]]
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_15178256}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 15178256 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_15178256}}
-==About this Structure==
-RGJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1p67 1p67]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RGJ OCA].
-==Reference==
-NMR and MD studies on the interaction between ligand peptides and alpha-bungarotoxin., Bernini A, Ciutti A, Spiga O, Scarselli M, Klein S, Vannetti S, Bracci L, Lozzi L, Lelli B, Falciani C, Neri P, Niccolai N, J Mol Biol. 2004 Jun 18;339(5):1169-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15178256 15178256]
 [[Category: Bungarus multicinctus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bernini, A.]]
+[[Category: Bernini A]]
-[[Category: Bracci, L.]]
+[[Category: Bracci L]]
-[[Category: Ciutti, A.]]
+[[Category: Ciutti A]]
-[[Category: Lelli, B.]]
+[[Category: Lelli B]]
-[[Category: Lozzi, L.]]
+[[Category: Lozzi L]]
-[[Category: Neri, P.]]
+[[Category: Neri P]]
-[[Category: Niccolai, N.]]
+[[Category: Niccolai N]]
-[[Category: Scarselli, M.]]
+[[Category: Scarselli M]]
-[[Category: Spiga, O.]]
+[[Category: Spiga O]]
-[[Category: Alpha-bungarotoxin]]
-[[Category: Beta-strand]]
-[[Category: Neurotoxin]]
-[[Category: Protein-peptide complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:47:45 2008''