1rfs: Difference between revisions

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[[Image:1rfs.jpg|left|200px]]


{{Structure
==RIESKE SOLUBLE FRAGMENT FROM SPINACH==
|PDB= 1rfs |SIZE=350|CAPTION= <scene name='initialview01'>1rfs</scene>, resolution 1.83&Aring;
<StructureSection load='1rfs' size='340' side='right'caption='[[1rfs]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
|SITE= <scene name='pdbsite=FSB:Fe-S+Binding+Site'>FSB</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>
<table><tr><td colspan='2'>[[1rfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RFS FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rfs OCA], [https://pdbe.org/1rfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rfs RCSB], [https://www.ebi.ac.uk/pdbsum/1rfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rfs ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rfs OCA], [http://www.ebi.ac.uk/pdbsum/1rfs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rfs RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/UCRIA_SPIOL UCRIA_SPIOL] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions (By similarity).
 
== Evolutionary Conservation ==
'''RIESKE SOLUBLE FRAGMENT FROM SPINACH'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/1rfs_consurf.spt"</scriptWhenChecked>
BACKGROUND: The cytochrome b6f complex functions in oxygenic photosynthesis as an integral membrane protein complex that mediates coupled electron transfer and proton translocation. The Rieske [2Fe-2S] protein subunit of the complex functions at the electropositive (p) membrane interface as the electron acceptor for plastoquinol and donor for the cytochrome f subunit, and may have a dynamic role in catalyzing electron and proton transfer at the membrane interface. There are significant structure/function similarities to the cytochrome bc1 complex of the respiratory chain. RESULTS: The 1.83 A crystal structure of a 139-residue C-terminal fragment of the Rieske [2Fe-2S] protein, derived from the cytochrome b6f complex of spinach chloroplasts, has been solved by multiwavelength anomalous diffraction. The structure of the fragment comprises two domains: a small 'cluster-binding' subdomain and a large subdomain. The [2Fe-2S] cluster-binding subdomains of the chloroplast and mitochondrial Rieske proteins are virtually identical, whereas the large subdomains are strikingly different despite a common folding topology. A structure-based sequence alignment of the b6f and bc1 groups of Rieske soluble domains is presented. CONCLUSIONS: The segregation of structural conservation and divergence in the cluster-binding and large subdomains of the Rieske protein correlates with the overall relatedness of the cytochrome b6f and bc1 complexes, in which redox domains in the aqueous p phase are dissimilar and those within the membrane are similar. Distinct sequences and surface charge distributions among Rieske large subdomains may provide a signature for interaction with the p-side oxidant protein and for the pH of the intraorganelle compartment.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1RFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFS OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rfs ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein., Carrell CJ, Zhang H, Cramer WA, Smith JL, Structure. 1997 Dec 15;5(12):1613-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9438861 9438861]
</StructureSection>
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
[[Category: Carrell, C J.]]
[[Category: Carrell CJ]]
[[Category: Cramer, W A.]]
[[Category: Cramer WA]]
[[Category: Smith, J L.]]
[[Category: Smith JL]]
[[Category: Zhang, H.]]
[[Category: Zhang H]]
[[Category: electron transport]]
[[Category: iron-sulfur protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:27:33 2008''

Latest revision as of 11:22, 1 May 2024

RIESKE SOLUBLE FRAGMENT FROM SPINACHRIESKE SOLUBLE FRAGMENT FROM SPINACH

Structural highlights

1rfs is a 1 chain structure with sequence from Spinacia oleracea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.83Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UCRIA_SPIOL Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1rfs, resolution 1.83Å

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