7qx7: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7qx7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_israelensis Bacillus thuringiensis serovar israelensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QX7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[7qx7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_israelensis Bacillus thuringiensis serovar israelensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QX7 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qx7 OCA], [https://pdbe.org/7qx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qx7 RCSB], [https://www.ebi.ac.uk/pdbsum/7qx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qx7 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qx7 OCA], [https://pdbe.org/7qx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qx7 RCSB], [https://www.ebi.ac.uk/pdbsum/7qx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qx7 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/C11AA_BACTI C11AA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.
[https://www.uniprot.org/uniprot/C11AA_BACTI C11AA_BACTI] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.
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== Publication Abstract from PubMed ==
Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.


De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals.,Tetreau G, Sawaya MR, De Zitter E, Andreeva EA, Banneville AS, Schibrowsky NA, Coquelle N, Brewster AS, Grunbein ML, Kovacs GN, Hunter MS, Kloos M, Sierra RG, Schiro G, Qiao P, Stricker M, Bideshi D, Young ID, Zala N, Engilberge S, Gorel A, Signor L, Teulon JM, Hilpert M, Foucar L, Bielecki J, Bean R, de Wijn R, Sato T, Kirkwood H, Letrun R, Batyuk A, Snigireva I, Fenel D, Schubert R, Canfield EJ, Alba MM, Laporte F, Despres L, Bacia M, Roux A, Chapelle C, Riobe F, Maury O, Ling WL, Boutet S, Mancuso A, Gutsche I, Girard E, Barends TRM, Pellequer JL, Park HW, Laganowsky AD, Rodriguez J, Burghammer M, Shoeman RL, Doak RB, Weik M, Sauter NK, Federici B, Cascio D, Schlichting I, Colletier JP Nat Commun. 2022 Jul 28;13(1):4376. doi: 10.1038/s41467-022-31746-x. PMID:35902572<ref>PMID:35902572</ref>
==See Also==
 
*[[Pesticidal crystal protein|Pesticidal crystal protein]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qx7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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Latest revision as of 10:54, 1 May 2024

mosquitocidal Cry11Aa-F17Y determined at pH 7 from naturally-occurring nanocrystals by Serial femtosecond crystallographymosquitocidal Cry11Aa-F17Y determined at pH 7 from naturally-occurring nanocrystals by Serial femtosecond crystallography

Structural highlights

7qx7 is a 1 chain structure with sequence from Bacillus thuringiensis serovar israelensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C11AA_BACTI Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.

See Also

7qx7, resolution 3.40Å

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OCA
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