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==Molecular Mechanism of ATP versus GTP Selectivity of Adenylate Kinase==
==Molecular Mechanism of ATP versus GTP Selectivity of Adenylate Kinase==
<StructureSection load='6f7u' size='340' side='right' caption='[[6f7u]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
<StructureSection load='6f7u' size='340' side='right'caption='[[6f7u]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6f7u]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F7U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F7U FirstGlance]. <br>
<table><tr><td colspan='2'>[[6f7u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F7U FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] </span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f7u OCA], [http://pdbe.org/6f7u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f7u RCSB], [http://www.ebi.ac.uk/pdbsum/6f7u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f7u ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f7u OCA], [https://pdbe.org/6f7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f7u RCSB], [https://www.ebi.ac.uk/pdbsum/6f7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f7u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/KAD_ECO55 KAD_ECO55]] Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
[https://www.uniprot.org/uniprot/KAD_ECOLI KAD_ECOLI] Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in the energy metabolism and nucleotide synthesis, is essential for maintenance and cell growth.[HAMAP-Rule:MF_00235]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzymatic substrate selectivity is critical for the precise control of metabolic pathways. In cases where chemically related substrates are present inside cells, robust mechanisms of substrate selectivity are required. Here, we report the mechanism utilized for catalytic ATP versus GTP selectivity during adenylate kinase (Adk) -mediated phosphorylation of AMP. Using NMR spectroscopy we found that while Adk adopts a catalytically competent and closed structural state in complex with ATP, the enzyme is arrested in a catalytically inhibited and open state in complex with GTP. X-ray crystallography experiments revealed that the interaction interfaces supporting ATP and GTP recognition, in part, are mediated by coinciding residues. The mechanism provides an atomic view on how the cellular GTP pool is protected from Adk turnover, which is important because GTP has many specialized cellular functions. In further support of this mechanism, a structure-function analysis enabled by synthesis of ATP analogs suggests that a hydrogen bond between the adenine moiety and the backbone of the enzyme is vital for ATP selectivity. The importance of the hydrogen bond for substrate selectivity is likely general given the conservation of its location and orientation across the family of eukaryotic protein kinases.


Molecular mechanism of ATP versus GTP selectivity of adenylate kinase.,Rogne P, Rosselin M, Grundstrom C, Hedberg C, Sauer UH, Wolf-Watz M Proc Natl Acad Sci U S A. 2018 Mar 5. pii: 1721508115. doi:, 10.1073/pnas.1721508115. PMID:29507216<ref>PMID:29507216</ref>
==See Also==
 
*[[Adenylate kinase 3D structures|Adenylate kinase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6f7u" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Adenylate kinase]]
[[Category: Escherichia coli K-12]]
[[Category: Grundstrom, C]]
[[Category: Large Structures]]
[[Category: Hedberg, C]]
[[Category: Grundstrom C]]
[[Category: Rogne, P]]
[[Category: Hedberg C]]
[[Category: Rosselin, M]]
[[Category: Rogne P]]
[[Category: Sauer, U H]]
[[Category: Rosselin M]]
[[Category: Wolf-Watz, M]]
[[Category: Sauer UH]]
[[Category: Atp selectivity]]
[[Category: Wolf-Watz M]]
[[Category: Gtp inhibition]]
[[Category: Inhibitor complex]]
[[Category: Transferase]]

Latest revision as of 10:32, 1 May 2024

Molecular Mechanism of ATP versus GTP Selectivity of Adenylate KinaseMolecular Mechanism of ATP versus GTP Selectivity of Adenylate Kinase

Structural highlights

6f7u is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.4Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KAD_ECOLI Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in the energy metabolism and nucleotide synthesis, is essential for maintenance and cell growth.[HAMAP-Rule:MF_00235]

See Also

6f7u, resolution 1.40Å

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