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| ==Superoxide reductase (1Fe-SOR) from Giardia intestinalis== | | ==Superoxide reductase (1Fe-SOR) from Giardia intestinalis== |
| <StructureSection load='4d7p' size='340' side='right' caption='[[4d7p]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='4d7p' size='340' side='right'caption='[[4d7p]], [[Resolution|resolution]] 2.00Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4d7p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giain Giain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D7P FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4d7p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D7P FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.001Å</td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Superoxide_reductase Superoxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.2 1.15.1.2] </span></td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d7p OCA], [http://pdbe.org/4d7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d7p RCSB], [http://www.ebi.ac.uk/pdbsum/4d7p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d7p ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d7p OCA], [https://pdbe.org/4d7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d7p RCSB], [https://www.ebi.ac.uk/pdbsum/4d7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d7p ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/V6TJK7_GIAIN V6TJK7_GIAIN] |
| Superoxide reductase (SOR), which is commonly found in prokaryotic organisms, affords protection from oxidative stress by reducing the superoxide anion to hydrogen peroxide. The reaction is catalyzed at the iron centre, which is highly conserved among the prokaryotic SORs structurally characterized to date. Reported here is the first structure of an SOR from a eukaryotic organism, the protozoan parasite Giardia intestinalis (GiSOR), which was solved at 2.0 A resolution. By collecting several diffraction data sets at 100 K from the same flash-cooled protein crystal using synchrotron X-ray radiation, photoreduction of the iron centre was observed. Reduction was monitored using an online UV-visible microspectrophotometer, following the decay of the 647 nm absorption band characteristic of the iron site in the glutamate-bound, oxidized state. Similarly to other 1Fe-SORs structurally characterized to date, the enzyme displays a tetrameric quaternary-structure arrangement. As a distinctive feature, the N-terminal loop of the protein, containing the characteristic EKHxP motif, revealed an unusually high flexibility regardless of the iron redox state. At variance with previous evidence collected by X-ray crystallography and Fourier transform infrared spectroscopy of prokaryotic SORs, iron reduction did not lead to dissociation of glutamate from the catalytic metal or other structural changes; however, the glutamate ligand underwent X-ray-induced chemical changes, revealing high sensitivity of the GiSOR active site to X-ray radiation damage.
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| Superoxide reductase from Giardia intestinalis: structural characterization of the first SOR from a eukaryotic organism shows an iron centre that is highly sensitive to photoreduction.,Sousa CM, Carpentier P, Matias PM, Testa F, Pinho F, Sarti P, Giuffre A, Bandeiras TM, Romao CV Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2236-47. doi:, 10.1107/S1399004715015825. Epub 2015 Oct 31. PMID:26527141<ref>PMID:26527141</ref>
| | ==See Also== |
| | | *[[Superoxide Reductase|Superoxide Reductase]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4d7p" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Giain]] | | [[Category: Giardia intestinalis]] |
| [[Category: Superoxide reductase]] | | [[Category: Large Structures]] |
| [[Category: Bandeiras, T M]] | | [[Category: Bandeiras TM]] |
| [[Category: Carpentier, P]] | | [[Category: Carpentier P]] |
| [[Category: Giuffre, A]] | | [[Category: Giuffre A]] |
| [[Category: Matias, P M]] | | [[Category: Matias PM]] |
| [[Category: Pinho, F G]] | | [[Category: Pinho FG]] |
| [[Category: Romao, C V]] | | [[Category: Romao CV]] |
| [[Category: Sarti, P]] | | [[Category: Sarti P]] |
| [[Category: Sousa, C M]] | | [[Category: Sousa CM]] |
| [[Category: Testa, F]] | | [[Category: Testa F]] |
| [[Category: Eukaryota]]
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| [[Category: Oxidative stress]]
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| [[Category: Oxidoreductase]]
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| [[Category: Parasite]]
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| [[Category: Pathogen]]
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| [[Category: Protozoan]]
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