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==The Structure of Serratia marcescens Lip, a membrane bound component of the Type VI Secretion System.==
==The Structure of Serratia marcescens Lip, a membrane bound component of the Type VI Secretion System.==
<StructureSection load='4a1r' size='340' side='right' caption='[[4a1r]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
<StructureSection load='4a1r' size='340' side='right'caption='[[4a1r]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4a1r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_marcescens"_(bizio_1823)_trevisan_in_de_toni_and_trevisan_1889 "bacillus marcescens" (bizio 1823) trevisan in de toni and trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A1R FirstGlance]. <br>
<table><tr><td colspan='2'>[[4a1r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A1R FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a1r OCA], [http://pdbe.org/4a1r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a1r RCSB], [http://www.ebi.ac.uk/pdbsum/4a1r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a1r ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a1r OCA], [https://pdbe.org/4a1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a1r RCSB], [https://www.ebi.ac.uk/pdbsum/4a1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a1r ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/G5EA77_SERMA G5EA77_SERMA]
Lip is a membrane-bound lipoprotein and a core component of the type VI secretion system found in Gram-negative bacteria. The structure of a Lip construct (residues 29-176) from Serratia marcescens (SmLip) has been determined at 1.92 A resolution. Experimental phases were derived using a single-wavelength anomalous dispersion approach on a sample cocrystallized with iodide. The membrane localization of the native protein was confirmed. The structure is that of the globular domain lacking only the lipoprotein signal peptide and the lipidated N-terminus of the mature protein. The protein fold is dominated by an eight-stranded beta-sandwich and identifies SmLip as a new member of the transthyretin family of proteins. Transthyretin and the only other member of the family fold, 5-hydroxyisourate hydrolase, form homotetramers important for their function. The asymmetric unit of SmLip is a tetramer with 222 symmetry, but the assembly is distinct from that previously noted for the transthyretin protein family. However, structural comparisons and bacterial two-hybrid data suggest that the SmLip tetramer is not relevant to its role as a core component of the type VI secretion system, but rather reflects a propensity for SmLip to participate in protein-protein interactions. A relatively low level of sequence conservation amongst Lip homologues is noted and is restricted to parts of the structure that might be involved in interactions with physiological partners.
 
The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system.,Rao VA, Shepherd SM, English G, Coulthurst SJ, Hunter WN Acta Crystallogr D Biol Crystallogr. 2011 Dec;67(Pt 12):1065-72. Epub 2011 Nov, 18. PMID:22120744<ref>PMID:22120744</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4a1r" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Coulthurst, S J]]
[[Category: Large Structures]]
[[Category: English, G]]
[[Category: Serratia marcescens]]
[[Category: Hunter, W N]]
[[Category: Coulthurst SJ]]
[[Category: Rao, V A]]
[[Category: English G]]
[[Category: Shepherd, S M]]
[[Category: Hunter WN]]
[[Category: Beta-sandwich]]
[[Category: Rao VA]]
[[Category: Membrane protein]]
[[Category: Shepherd SM]]
[[Category: T6ss]]

Latest revision as of 10:13, 1 May 2024

The Structure of Serratia marcescens Lip, a membrane bound component of the Type VI Secretion System.The Structure of Serratia marcescens Lip, a membrane bound component of the Type VI Secretion System.

Structural highlights

4a1r is a 4 chain structure with sequence from Serratia marcescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.92Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G5EA77_SERMA

4a1r, resolution 1.92Å

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