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==Structure of Reduced M Smegmatis 5246, a homologue of M.Tuberculosis Acg==
==Structure of Reduced M Smegmatis 5246, a homologue of M.Tuberculosis Acg==
<StructureSection load='2ymv' size='340' side='right' caption='[[2ymv]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='2ymv' size='340' side='right'caption='[[2ymv]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ymv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_smegmatis"_trevisan_1889 "bacillus smegmatis" trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YMV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YMV FirstGlance]. <br>
<table><tr><td colspan='2'>[[2ymv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YMV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=FNR:1-DEOXY-1-(7,8-DIMETHYL-2,4-DIOXO-3,4-DIHYDRO-2H-BENZO[G]PTERIDIN-1-ID-10(5H)-YL)-5-O-PHOSPHONATO-D-RIBITOL'>FNR</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=FNR:1-DEOXY-1-(7,8-DIMETHYL-2,4-DIOXO-3,4-DIHYDRO-2H-BENZO[G]PTERIDIN-1-ID-10(5H)-YL)-5-O-PHOSPHONATO-D-RIBITOL'>FNR</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ymv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ymv OCA], [http://pdbe.org/2ymv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ymv RCSB], [http://www.ebi.ac.uk/pdbsum/2ymv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ymv ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ymv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ymv OCA], [https://pdbe.org/2ymv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ymv RCSB], [https://www.ebi.ac.uk/pdbsum/2ymv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ymv ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0R2V4_MYCS2 A0R2V4_MYCS2]
This paper presents the structure of MsAcg (MSMEG_5246), a Mycobacterium smegmatis homologue of Mycobacterium tuberculosis Acg (Rv2032) in its reduced form at 1.6 A resolution using x-ray crystallography. Rv2032 is one of the most induced genes under the hypoxic model of tuberculosis dormancy. The Acg family turns out to be unusual flavin mononucleotide (FMN)-binding proteins that have probably arisen by gene duplication and fusion from a classical homodimeric nitroreductase such that the monomeric protein resembles a classical nitroreductase dimer but with one active site deleted and the other active site covered by a unique lid. The FMN cofactor is not reduced by either NADH or NADPH, but the chemically reduced enzyme is capable of reduction of nitro substrates, albeit at no kinetic advantage over free FMN. The reduced enzyme is rapidly oxidized by oxygen but without any evidence for a radical state commonly seen in oxygen-sensitive nitroreductases. The presence of the unique lid domain, the lack of reduction by NAD(P)H, and the slow rate of reaction of the chemically reduced protein raises a possible alternative function of Acg proteins in FMN storage or sequestration from other biochemical pathways as part of the bacteria's adaptation to a dormancy state.
 
Crystal Structure of Reduced MsAcg, a Putative Nitroreductase from Mycobacterium smegmatis and a Close Homologue of Mycobacterium tuberculosis Acg.,Chauviac FX, Bommer M, Yan J, Parkin G, Daviter T, Lowden P, Raven EL, Thalassinos K, Keep NH J Biol Chem. 2012 Dec 28;287(53):44372-83. doi: 10.1074/jbc.M112.406264. Epub, 2012 Nov 12. PMID:23148223<ref>PMID:23148223</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ymv" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Nitroreductase|Nitroreductase]]
*[[Nitroreductase 3D structures|Nitroreductase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus smegmatis trevisan 1889]]
[[Category: Large Structures]]
[[Category: Bommer, M]]
[[Category: Mycolicibacterium smegmatis]]
[[Category: Chauviac, F X]]
[[Category: Bommer M]]
[[Category: Dobbek, H]]
[[Category: Chauviac F-X]]
[[Category: Keep, N H]]
[[Category: Dobbek H]]
[[Category: Dormancy]]
[[Category: Keep NH]]
[[Category: Oxidoreductase]]
[[Category: Reduced fmn]]

Latest revision as of 10:12, 1 May 2024

Structure of Reduced M Smegmatis 5246, a homologue of M.Tuberculosis AcgStructure of Reduced M Smegmatis 5246, a homologue of M.Tuberculosis Acg

Structural highlights

2ymv is a 1 chain structure with sequence from Mycolicibacterium smegmatis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:, , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0R2V4_MYCS2

See Also

2ymv, resolution 1.60Å

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