2x2s: Difference between revisions

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 ==Crystal structure of Sclerotinia sclerotiorum agglutinin SSA==
-<StructureSection load='2x2s' size='340' side='right' caption='[[2x2s]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+<StructureSection load='2x2s' size='340' side='right'caption='[[2x2s]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2x2s]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sclerotinia_sclerotiorum Sclerotinia sclerotiorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X2S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2x2s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sclerotinia_sclerotiorum Sclerotinia sclerotiorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X2S FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x2t|2x2t]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2s OCA], [http://pdbe.org/2x2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x2s RCSB], [http://www.ebi.ac.uk/pdbsum/2x2s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2s ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2s OCA], [https://pdbe.org/2x2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x2s RCSB], [https://www.ebi.ac.uk/pdbsum/2x2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2s ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/AGGL_SCLSC AGGL_SCLSC] Lectin that primarily recognizes glycans with a non-reducing terminal N-acetylgalactosamine (GalNAc), with a preference for the alpha- over the beta-anomer. Can also bind non-reducing terminal galactose (Gal) residues but with a lower affinity. Strongly interacts with glycolipid type glycans with terminal non-reducing Gal or GalNAc but fails to bind sialylated or fucosylated forms of the same glycans. Strongly interacts with galactosylated N-glycans, displaying highest affinity for alpha-1-3 branched mono-antennary N-glycans but also binding to multi-antennary glycans.<ref>PMID:12901882</ref> <ref>PMID:17294128</ref> <ref>PMID:20566411</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
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 </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x2s ConSurf].
 <div style="clear:both"></div>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-A lectin from the phytopathogenic ascomycete Sclerotinia sclerotiorum that shares only weak sequence similarity with characterized fungal lectins has recently been identified. S. sclerotiorum agglutinin (SSA) is a homodimeric protein consisting of two identical subunits of approximately 17 kDa and displays specificity primarily towards Gal/GalNAc. Glycan array screening indicates that SSA readily interacts with Gal/GalNAc-bearing glycan chains. The crystal structures of SSA in the ligand-free form and in complex with the Gal-beta1,3-GalNAc (T-antigen) disaccharide have been determined at 1.6 and 1.97 A resolution, respectively. SSA adopts a beta-trefoil domain as previously identified for other carbohydrate-binding proteins of the ricin B-like lectin superfamily and accommodates terminal non-reducing galactosyl and N-acetylgalactosaminyl glycans. Unlike other structurally related lectins, SSA contains a single carbohydrate-binding site at site alpha. SSA reveals a novel dimeric assembly markedly dissimilar to those described earlier for ricin-type lectins. The present structure exemplifies the adaptability of the beta-trefoil domain in the evolution of fungal lectins.
-Crystal structure of the GalNAc/Gal-specific agglutinin from the phytopathogenic ascomycete Sclerotinia sclerotiorum reveals novel adaptation of a beta-trefoil domain.,Sulzenbacher G, Roig-Zamboni V, Peumans WJ, Rouge P, Van Damme EJ, Bourne Y J Mol Biol. 2010 Jul 23;400(4):715-23. Epub 2010 May 24. PMID:20566411<ref>PMID:20566411</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 2x2s" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[Agglutinin|Agglutinin]]
+*[[Agglutinin 3D structures|Agglutinin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Sclerotinia sclerotiorum]]
-[[Category: Bourne, Y]]
+[[Category: Bourne Y]]
-[[Category: Damme, E J.M Van]]
+[[Category: Peumans WJ]]
-[[Category: Peumans, W J]]
+[[Category: Roig-Zamboni V]]
-[[Category: Roig-Zamboni, V]]
+[[Category: Rouge P]]
-[[Category: Rouge, P]]
+[[Category: Sulzenbacher G]]
-[[Category: Sulzenbacher, G]]
+[[Category: Van Damme EJM]]
-[[Category: Beta-trefoil domain]]
-[[Category: Cell adhesion]]
-[[Category: Fungal lectin]]