2vel: Difference between revisions
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==Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties== | |||
<StructureSection load='2vel' size='340' side='right'caption='[[2vel]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
=== | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2vel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VEL FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PGA:2-PHOSPHOGLYCOLIC+ACID'>PGA</scene></td></tr> | |||
== | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vel OCA], [https://pdbe.org/2vel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vel RCSB], [https://www.ebi.ac.uk/pdbsum/2vel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vel ProSAT]</span></td></tr> | ||
[[2vel]] is a 2 chain structure | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/TPIS_TRYBB TPIS_TRYBB] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ve/2vel_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vel ConSurf]. | |||
<div style="clear:both"></div> | |||
==See Also== | ==See Also== | ||
*[[Triose | *[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]] | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: | |||
[[Category: Trypanosoma brucei brucei]] | [[Category: Trypanosoma brucei brucei]] | ||
[[Category: Alahuhta | [[Category: Alahuhta M]] | ||
[[Category: Augustyns | [[Category: Augustyns K]] | ||
[[Category: Casteleijn | [[Category: Casteleijn MG]] | ||
[[Category: El-Sayed | [[Category: El-Sayed I]] | ||
[[Category: Kemmer | [[Category: Kemmer C]] | ||
[[Category: Neubauer | [[Category: Neubauer P]] | ||
[[Category: Salin | [[Category: Salin M]] | ||
[[Category: Wierenga | [[Category: Wierenga RK]] | ||
Latest revision as of 10:07, 1 May 2024
Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding propertiesStructure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. See Also |
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