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{{Large structure}}
 
==The external thioesterase of the Surfactin-Synthetase==
==The external thioesterase of the Surfactin-Synthetase==
<StructureSection load='2ron' size='340' side='right' caption='[[2ron]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2ron' size='340' side='right'caption='[[2ron]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ron]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RON OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RON FirstGlance]. <br>
<table><tr><td colspan='2'>[[2ron]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RON FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">srfAD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ron FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ron OCA], [http://pdbe.org/2ron PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ron RCSB], [http://www.ebi.ac.uk/pdbsum/2ron PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ron ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ron FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ron OCA], [https://pdbe.org/2ron PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ron RCSB], [https://www.ebi.ac.uk/pdbsum/2ron PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ron ProSAT]</span></td></tr>
</table>
</table>
{{Large structure}}
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SRFAD_BACSU SRFAD_BACSU]] Probable thioesterase involved in the biosynthesis of surfactin.  
[https://www.uniprot.org/uniprot/SRFAD_BACSU SRFAD_BACSU] Probable thioesterase involved in the biosynthesis of surfactin.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ron ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ron ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) found in bacteria, fungi and plants use two different types of thioesterases for the production of highly active biological compounds. Type I thioesterases (TEI) catalyse the release step from the assembly line of the final product where it is transported from one reaction centre to the next as a thioester linked to a 4'-phosphopantetheine (4'-PP) cofactor that is covalently attached to thiolation (T) domains. The second enzyme involved in the synthesis of these secondary metabolites, the type II thioesterase (TEII), is a crucial repair enzyme for the regeneration of functional 4'-PP cofactors of holo-T domains of NRPS and PKS systems. Mispriming of 4'-PP cofactors by acetyl- and short-chain acyl-residues interrupts the biosynthetic system. This repair reaction is very important, because roughly 80% of CoA, the precursor of the 4'-PP cofactor, is acetylated in bacteria. Here we report the three-dimensional structure of a type II thioesterase from Bacillus subtilis free and in complex with a T domain. Comparison with structures of TEI enzymes shows the basis for substrate selectivity and the different modes of interaction of TEII and TEI enzymes with T domains. Furthermore, we show that the TEII enzyme exists in several conformations of which only one is selected on interaction with its native substrate, a modified holo-T domain.
Structural basis for the selectivity of the external thioesterase of the surfactin synthetase.,Koglin A, Lohr F, Bernhard F, Rogov VV, Frueh DP, Strieter ER, Mofid MR, Guntert P, Wagner G, Walsh CT, Marahiel MA, Dotsch V Nature. 2008 Aug 14;454(7206):907-11. PMID:18704089<ref>PMID:18704089</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ron" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Vibrio subtilis ehrenberg 1835]]
[[Category: Bacillus subtilis]]
[[Category: Bernhard, F]]
[[Category: Large Structures]]
[[Category: Doetsch, V]]
[[Category: Bernhard F]]
[[Category: Frueh, D P]]
[[Category: Doetsch V]]
[[Category: Guentert, P]]
[[Category: Frueh DP]]
[[Category: Koglin, A]]
[[Category: Guentert P]]
[[Category: Lohr, F]]
[[Category: Koglin A]]
[[Category: Marahiel, M A]]
[[Category: Lohr F]]
[[Category: Mofid, M R]]
[[Category: Marahiel MA]]
[[Category: Rogov, V V]]
[[Category: Mofid MR]]
[[Category: Strieter, E R]]
[[Category: Rogov VV]]
[[Category: Wagner, G]]
[[Category: Strieter ER]]
[[Category: Walsh, C T]]
[[Category: Wagner G]]
[[Category: A/b hydrolase]]
[[Category: Walsh CT]]
[[Category: Antibiotic biosynthesis]]
[[Category: Hydrolase]]
[[Category: Non-ribosomal peptide synthetase]]
[[Category: Nrp]]
[[Category: Pcp regeneration]]
[[Category: Sporulation]]
[[Category: Stress response]]
[[Category: Teii]]
[[Category: Thioesterase]]

Latest revision as of 10:05, 1 May 2024

The external thioesterase of the Surfactin-SynthetaseThe external thioesterase of the Surfactin-Synthetase

Structural highlights

2ron is a 1 chain structure with sequence from Bacillus subtilis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SRFAD_BACSU Probable thioesterase involved in the biosynthesis of surfactin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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