2rol: Difference between revisions

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==Structural Basis of PxxDY motif recognition in SH3 binding==
==Structural Basis of PxxDY motif recognition in SH3 binding==
<StructureSection load='2rol' size='340' side='right'caption='[[2rol]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2rol' size='340' side='right'caption='[[2rol]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2rol]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ROL FirstGlance]. <br>
<table><tr><td colspan='2'>[[2rol]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ROL FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EPS8L1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rol OCA], [https://pdbe.org/2rol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rol RCSB], [https://www.ebi.ac.uk/pdbsum/2rol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rol ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rol OCA], [https://pdbe.org/2rol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rol RCSB], [https://www.ebi.ac.uk/pdbsum/2rol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rol ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/ES8L1_HUMAN ES8L1_HUMAN]] Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.<ref>PMID:14565974</ref> [[https://www.uniprot.org/uniprot/CD3E_HUMAN CD3E_HUMAN]] The CD3 complex mediates signal transduction.
[https://www.uniprot.org/uniprot/ES8L1_HUMAN ES8L1_HUMAN] Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.<ref>PMID:14565974</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rol ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rol ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have determined the solution structure of epidermal growth factor receptor pathway substrate 8 (Eps8) L1 Src homology 3 (SH3) domain in complex with the PPVPNPDYEPIR peptide from the CD3epsilon cytoplasmic tail. Our structure reveals the distinct structural features that account for the unusual specificity of the Eps8 family SH3 domains for ligands containing a PxxDY motif instead of canonical PxxP ligands. The CD3epsilon peptide binds Eps8L1 SH3 in a class II orientation, but neither adopts a polyproline II helical conformation nor engages the first proline-binding pocket of the SH3 ligand binding interface. Ile531 of Eps8L1 SH3, instead of Tyr or Phe residues typically found in this position in SH3 domains, renders this hydrophobic pocket smaller and nonoptimal for binding to conventional PxxP peptides. A positively charged arginine at position 512 in the n-Src loop of Eps8L1 SH3 plays a key role in PxxDY motif recognition by forming a salt bridge to D7 of the CD3epsilon peptide. In addition, our structural model suggests a hydrogen bond between the hydroxyl group of the aromatic ring of Y8 and the carboxyl group of E496, thus explaining the critical role of the PxxDY motif tyrosine residue in binding to Eps8 family SH3. These finding have direct implications also for understanding the atypical binding specificity of the amino-terminal SH3 of the Nck family proteins.
Structural basis of PxxDY motif recognition in SH3 binding.,Aitio O, Hellman M, Kesti T, Kleino I, Samuilova O, Paakkonen K, Tossavainen H, Saksela K, Permi P J Mol Biol. 2008 Sep 26;382(1):167-78. Epub 2008 Jul 11. PMID:18644376<ref>PMID:18644376</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2rol" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Aitio, O]]
[[Category: Aitio O]]
[[Category: Hellman, M]]
[[Category: Hellman M]]
[[Category: Kesti, T]]
[[Category: Kesti T]]
[[Category: Kleino, I]]
[[Category: Kleino I]]
[[Category: Paakkonen, K]]
[[Category: Paakkonen K]]
[[Category: Permi, P]]
[[Category: Permi P]]
[[Category: Saksela, K]]
[[Category: Saksela K]]
[[Category: Samuilova, O]]
[[Category: Samuilova O]]
[[Category: Tossavainen, H]]
[[Category: Tossavainen H]]
[[Category: Alternative splicing]]
[[Category: Cd3e]]
[[Category: Coiled coil]]
[[Category: Complex structure]]
[[Category: Cytoplasm]]
[[Category: Eps8l1]]
[[Category: Immunoglobulin domain]]
[[Category: Membrane]]
[[Category: Phosphoprotein]]
[[Category: Receptor]]
[[Category: Sh3]]
[[Category: Sh3 domain]]
[[Category: Splicing-signaling protein complex]]
[[Category: Transmembrane]]

Latest revision as of 10:05, 1 May 2024

Structural Basis of PxxDY motif recognition in SH3 bindingStructural Basis of PxxDY motif recognition in SH3 binding

Structural highlights

2rol is a 2 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ES8L1_HUMAN Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Offenhauser N, Borgonovo A, Disanza A, Romano P, Ponzanelli I, Iannolo G, Di Fiore PP, Scita G. The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway. Mol Biol Cell. 2004 Jan;15(1):91-8. Epub 2003 Oct 17. PMID:14565974 doi:http://dx.doi.org/10.1091/mbc.E03-06-0427
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