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==An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES==
==An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES==
<StructureSection load='2n8v' size='340' side='right' caption='[[2n8v]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='2n8v' size='340' side='right'caption='[[2n8v]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2n8v]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N8V FirstGlance]. <br>
<table><tr><td colspan='2'>[[2n8v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8V FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8v OCA], [http://pdbe.org/2n8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n8v RCSB], [http://www.ebi.ac.uk/pdbsum/2n8v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8v ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8v OCA], [https://pdbe.org/2n8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8v RCSB], [https://www.ebi.ac.uk/pdbsum/2n8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8v ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The dicistrovirus intergenic region internal ribosome entry site (IRES) adopts a triple-pseudoknotted RNA structure and occupies the core ribosomal E, P, and A sites to directly recruit the ribosome and initiate translation at a non-AUG codon. A subset of dicistrovirus IRESs directs translation in the 0 and +1 frames to produce the viral structural proteins and a +1 overlapping open reading frame called ORFx, respectively. Here we show that specific mutations of two unpaired adenosines located at the core of the three-helical junction of the honey bee dicistrovirus Israeli acute paralysis virus (IAPV) IRES PKI domain can uncouple 0 and +1 frame translation, suggesting that the structure adopts distinct conformations that contribute to 0 or +1 frame translation. Using a reconstituted translation system, we show that ribosomes assembled on mutant IRESs that direct exclusive 0 or +1 frame translation lack reading frame fidelity. Finally, a nuclear magnetic resonance/small-angle X-ray scattering hybrid approach reveals that the PKI domain of the IAPV IRES adopts an RNA structure that resembles a complete tRNA. The tRNA shape-mimicry enables the viral IRES to gain access to the ribosome tRNA-binding sites and form intermolecular contacts with the ribosome that are necessary for initiating IRES translation in a specific reading frame.
Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection.,Au HH, Cornilescu G, Mouzakis KD, Ren Q, Burke JE, Lee S, Butcher SE, Jan E Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):E6446-55. doi:, 10.1073/pnas.1512088112. Epub 2015 Nov 9. PMID:26554019<ref>PMID:26554019</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2n8v" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Au, H H]]
[[Category: Apis mellifera]]
[[Category: Burke, J E]]
[[Category: Large Structures]]
[[Category: Butcher, S E]]
[[Category: Au HH]]
[[Category: Cornilescu, G]]
[[Category: Burke JE]]
[[Category: Jan, E]]
[[Category: Butcher SE]]
[[Category: Lee, S]]
[[Category: Cornilescu G]]
[[Category: Mouzakis, K D]]
[[Category: Jan E]]
[[Category: Ren, Q]]
[[Category: Lee S]]
[[Category: Iapv]]
[[Category: Mouzakis KD]]
[[Category: Pseudoknot]]
[[Category: Ren Q]]
[[Category: Rna]]

Latest revision as of 10:04, 1 May 2024

An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRESAn NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES

Structural highlights

2n8v is a 1 chain structure with sequence from Apis mellifera. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT
Drag the structure with the mouse to rotate

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