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| ==An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES== | | ==An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES== |
| <StructureSection load='2n8v' size='340' side='right' caption='[[2n8v]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | | <StructureSection load='2n8v' size='340' side='right'caption='[[2n8v]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2n8v]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N8V FirstGlance]. <br> | | <table><tr><td colspan='2'>[[2n8v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8V FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8v OCA], [http://pdbe.org/2n8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n8v RCSB], [http://www.ebi.ac.uk/pdbsum/2n8v PDBsum]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8v OCA], [https://pdbe.org/2n8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8v RCSB], [https://www.ebi.ac.uk/pdbsum/2n8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8v ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The dicistrovirus intergenic region internal ribosome entry site (IRES) adopts a triple-pseudoknotted RNA structure and occupies the core ribosomal E, P, and A sites to directly recruit the ribosome and initiate translation at a non-AUG codon. A subset of dicistrovirus IRESs directs translation in the 0 and +1 frames to produce the viral structural proteins and a +1 overlapping open reading frame called ORFx, respectively. Here we show that specific mutations of two unpaired adenosines located at the core of the three-helical junction of the honey bee dicistrovirus Israeli acute paralysis virus (IAPV) IRES PKI domain can uncouple 0 and +1 frame translation, suggesting that the structure adopts distinct conformations that contribute to 0 or +1 frame translation. Using a reconstituted translation system, we show that ribosomes assembled on mutant IRESs that direct exclusive 0 or +1 frame translation lack reading frame fidelity. Finally, a nuclear magnetic resonance/small-angle X-ray scattering hybrid approach reveals that the PKI domain of the IAPV IRES adopts an RNA structure that resembles a complete tRNA. The tRNA shape-mimicry enables the viral IRES to gain access to the ribosome tRNA-binding sites and form intermolecular contacts with the ribosome that are necessary for initiating IRES translation in a specific reading frame.
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| Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection.,Au HH, Cornilescu G, Mouzakis KD, Ren Q, Burke JE, Lee S, Butcher SE, Jan E Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):E6446-55. doi:, 10.1073/pnas.1512088112. Epub 2015 Nov 9. PMID:26554019<ref>PMID:26554019</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2n8v" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Au, H H]] | | [[Category: Apis mellifera]] |
| [[Category: Burke, J E]] | | [[Category: Large Structures]] |
| [[Category: Butcher, S E]] | | [[Category: Au HH]] |
| [[Category: Cornilescu, G]] | | [[Category: Burke JE]] |
| [[Category: Jan, E]] | | [[Category: Butcher SE]] |
| [[Category: Lee, S]] | | [[Category: Cornilescu G]] |
| [[Category: Mouzakis, K D]] | | [[Category: Jan E]] |
| [[Category: Ren, Q]] | | [[Category: Lee S]] |
| [[Category: Iapv]] | | [[Category: Mouzakis KD]] |
| [[Category: Pseudoknot]] | | [[Category: Ren Q]] |
| [[Category: Rna]]
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