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==A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement==
==A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement==
<StructureSection load='2mc6' size='340' side='right'caption='[[2mc6]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
<StructureSection load='2mc6' size='340' side='right'caption='[[2mc6]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2mc6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris_phage_xp10 Xanthomonas campestris phage xp10]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MC6 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2mc6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_oryzae_pv._oryzae_KACC_10331 Xanthomonas oryzae pv. oryzae KACC 10331] and [https://en.wikipedia.org/wiki/Xanthomonas_virus_Xp10 Xanthomonas virus Xp10]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MC6 FirstGlance]. <br>
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc6 OCA], [https://pdbe.org/2mc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mc6 RCSB], [https://www.ebi.ac.uk/pdbsum/2mc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc6 OCA], [https://pdbe.org/2mc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mc6 RCSB], [https://www.ebi.ac.uk/pdbsum/2mc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc6 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/RPOC_XANOR RPOC_XANOR]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).
[https://www.uniprot.org/uniprot/Q8LTJ5_9CAUD Q8LTJ5_9CAUD]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic beta and beta' subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the sigma70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.
 
A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement.,Liu B, Shadrin A, Sheppard C, Mekler V, Xu Y, Severinov K, Matthews S, Wigneshweraraj S Nucleic Acids Res. 2014 Jan 30. PMID:24482445<ref>PMID:24482445</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mc6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: DNA-directed RNA polymerase]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Xanthomonas campestris phage xp10]]
[[Category: Xanthomonas oryzae pv. oryzae KACC 10331]]
[[Category: Liu, B]]
[[Category: Xanthomonas virus Xp10]]
[[Category: Matthews, S]]
[[Category: Liu B]]
[[Category: Severinov, K]]
[[Category: Matthews S]]
[[Category: Shadrin, A]]
[[Category: Severinov K]]
[[Category: Sheppard, C]]
[[Category: Shadrin A]]
[[Category: Wigneshweraraj, S]]
[[Category: Sheppard C]]
[[Category: Xu, Y]]
[[Category: Wigneshweraraj S]]
[[Category: Beta prime subunit]]
[[Category: Xu Y]]
[[Category: Ntd]]
[[Category: P7]]
[[Category: Rnap]]
[[Category: Sigma factor]]
[[Category: Transcription]]
[[Category: Transferase]]

Latest revision as of 09:59, 1 May 2024

A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacementA bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement

Structural highlights

2mc6 is a 2 chain structure with sequence from Xanthomonas oryzae pv. oryzae KACC 10331 and Xanthomonas virus Xp10. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8LTJ5_9CAUD

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OCA
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