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==Pre-Fusion Solution NMR Structure of Neuronal SNARE Syntaxin 1A==
==Pre-Fusion Solution NMR Structure of Neuronal SNARE Syntaxin 1A==
<StructureSection load='2m8r' size='340' side='right' caption='[[2m8r]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2m8r' size='340' side='right'caption='[[2m8r]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2m8r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. The November 2013 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''SNARE Proteins''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2013_11 10.2210/rcsb_pdb/mom_2013_11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M8R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M8R FirstGlance]. <br>
<table><tr><td colspan='2'>[[2m8r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The November 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''SNARE Proteins''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_11 10.2210/rcsb_pdb/mom_2013_11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M8R FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Stx1a, Sap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m8r OCA], [http://pdbe.org/2m8r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m8r RCSB], [http://www.ebi.ac.uk/pdbsum/2m8r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2m8r ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m8r OCA], [https://pdbe.org/2m8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m8r RCSB], [https://www.ebi.ac.uk/pdbsum/2m8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m8r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT]] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
[https://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The assembly of the three neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key step that leads to exocytotic fusion of synaptic vesicles. In the fully assembled SNARE complex, these three proteins form a coiled-coil four-helix bundle structure by interaction of their respective SNARE motifs. Although biochemical and mutational analyses strongly suggest that the heptad-repeat SNARE motifs zipper into the final structure, little is known about the prefusion state of individual membrane-bound SNAREs and how they change conformation from the unzippered prefusion to the zippered postfusion state in a membrane environment. We have solved the solution NMR structure of micelle-bound syntaxin-1A in its prefusion conformation. In addition to the transmembrane helix, the SNARE motif consists of two well-ordered, membrane-bound helices separated by the "0-layer" residue Gln226. This unexpected structural order of the N- and C-terminal halves of the uncomplexed SNARE motif suggests the formation of partially zippered SNARE complex intermediates, with the 0-layer serving as a proofreading site for correct SNARE assembly. Interferometric fluorescence measurements in lipid bilayers confirm that the open SNARE motif helices of syntaxin interact with lipid bilayers and that association with the other target-membrane SNARE SNAP-25 lifts the SNARE motif off the membrane as a critical prerequisite for SNARE complex assembly and membrane fusion.


Prefusion structure of syntaxin-1A suggests pathway for folding into neuronal trans-SNARE complex fusion intermediate.,Liang B, Kiessling V, Tamm LK Proc Natl Acad Sci U S A. 2013 Nov 11. PMID:24218570<ref>PMID:24218570</ref>
==See Also==
 
*[[Syntaxin 3D structures|Syntaxin 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2m8r" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Buffalo rat]]
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: SNARE Proteins]]
[[Category: SNARE Proteins]]
[[Category: Kiessling, V]]
[[Category: Kiessling V]]
[[Category: Liang, B]]
[[Category: Liang B]]
[[Category: Tamm, L K]]
[[Category: Tamm LK]]
[[Category: Membrane protein]]
[[Category: Prefusion]]
[[Category: Snare]]
[[Category: Syntaxin]]

Latest revision as of 09:59, 1 May 2024

Pre-Fusion Solution NMR Structure of Neuronal SNARE Syntaxin 1APre-Fusion Solution NMR Structure of Neuronal SNARE Syntaxin 1A

Structural highlights

2m8r is a 1 chain structure with sequence from Rattus norvegicus. The November 2013 RCSB PDB Molecule of the Month feature on SNARE Proteins by David Goodsell is 10.2210/rcsb_pdb/mom_2013_11. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

STX1A_RAT Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.

See Also

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