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| ==The solution structure of the Dm DCP1 EVH1 domain in complex with the XRN1 DBM peptide== | | ==The solution structure of the Dm DCP1 EVH1 domain in complex with the XRN1 DBM peptide== |
| <StructureSection load='2lyd' size='340' side='right' caption='[[2lyd]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''> | | <StructureSection load='2lyd' size='340' side='right'caption='[[2lyd]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2lyd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LYD FirstGlance]. <br> | | <table><tr><td colspan='2'>[[2lyd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LYD FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dcp1, CG11183, Dmel_CG11183 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), pcm, pacman, CG3291 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lyd OCA], [http://pdbe.org/2lyd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lyd RCSB], [http://www.ebi.ac.uk/pdbsum/2lyd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lyd ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lyd OCA], [https://pdbe.org/2lyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lyd RCSB], [https://www.ebi.ac.uk/pdbsum/2lyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lyd ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q9W1H5_DROME Q9W1H5_DROME] |
| The removal of the mRNA 5' cap structure by the decapping enzyme DCP2 leads to rapid 5'-->3' mRNA degradation by XRN1, suggesting that the two processes are coordinated, but the coupling mechanism is unknown. DCP2 associates with the decapping activators EDC4 and DCP1. Here we show that XRN1 directly interacts with EDC4 and DCP1 in human and Drosophila melanogaster cells, respectively. In D. melanogaster cells, this interaction is mediated by the DCP1 EVH1 domain and a DCP1-binding motif (DBM) in the XRN1 C-terminal region. The NMR structure of the DCP1 EVH1 domain bound to the DBM reveals that the peptide docks at a conserved aromatic cleft, which is used by EVH1 domains to recognize proline-rich ligands. Our findings reveal a role for XRN1 in decapping and provide a molecular basis for the coupling of decapping to 5'-->3' mRNA degradation.
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| A direct interaction between DCP1 and XRN1 couples mRNA decapping to 5' exonucleolytic degradation.,Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, Weichenrieder O, Coles M, Izaurralde E Nat Struct Mol Biol. 2012 Nov 11. doi: 10.1038/nsmb.2413. PMID:23142987<ref>PMID:23142987</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2lyd" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Drome]] | | [[Category: Drosophila melanogaster]] |
| [[Category: Truffault, V]] | | [[Category: Large Structures]] |
| [[Category: Dcp1]] | | [[Category: Truffault V]] |
| [[Category: Transcription-protein binding complex]]
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| [[Category: Xrn1]]
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