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==Solution NMR Structure of the uncharacterized protein from gene locus VNG_0733H of Halobacterium salinarium, Northeast Structural Genomics Consortium Target HsR50==
==Solution NMR Structure of the uncharacterized protein from gene locus VNG_0733H of Halobacterium salinarium, Northeast Structural Genomics Consortium Target HsR50==
<StructureSection load='2lok' size='340' side='right' caption='[[2lok]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2lok' size='340' side='right'caption='[[2lok]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2lok]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaea Archaea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LOK FirstGlance]. <br>
<table><tr><td colspan='2'>[[2lok]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum_NRC-1 Halobacterium salinarum NRC-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LOK FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VNG_0733H ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2157 Archaea])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lok OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lok RCSB], [http://www.ebi.ac.uk/pdbsum/2lok PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lok OCA], [https://pdbe.org/2lok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lok RCSB], [https://www.ebi.ac.uk/pdbsum/2lok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lok ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q9HRE7_HALSA Q9HRE7_HALSA]
We have developed an approach for determining NMR structures of proteins over 20 kDa that utilizes sparse distance restraints obtained using transverse relaxation optimized spectroscopy experiments on perdeuterated samples to guide RASREC Rosetta NMR structure calculations. The method was tested on 11 proteins ranging from 15 to 40 kDa, seven of which were previously unsolved. The RASREC Rosetta models were in good agreement with models obtained using traditional NMR methods with larger restraint sets. In five cases X-ray structures were determined or were available, allowing comparison of the accuracy of the Rosetta models and conventional NMR models. In all five cases, the Rosetta models were more similar to the X-ray structures over both the backbone and side-chain conformations than the "best effort" structures determined by conventional methods. The incorporation of sparse distance restraints into RASREC Rosetta allows routine determination of high-quality solution NMR structures for proteins up to 40 kDa, and should be broadly useful in structural biology.
 
Determination of solution structures of proteins up to 40 kDa using CS-Rosetta with sparse NMR data from deuterated samples.,Lange OF, Rossi P, Sgourakis NG, Song Y, Lee HW, Aramini JM, Ertekin A, Xiao R, Acton TB, Montelione GT, Baker D Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10873-8. Epub 2012 Jun 25. PMID:22733734<ref>PMID:22733734</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Archaea]]
[[Category: Halobacterium salinarum NRC-1]]
[[Category: Acton, T B]]
[[Category: Large Structures]]
[[Category: Buchwald, W A]]
[[Category: Acton TB]]
[[Category: Ciccosanti, C]]
[[Category: Buchwald WA]]
[[Category: Everett, J K]]
[[Category: Ciccosanti C]]
[[Category: Hamilton, K]]
[[Category: Everett JK]]
[[Category: Lange, O F]]
[[Category: Hamilton K]]
[[Category: Lee, H]]
[[Category: Lange OF]]
[[Category: Montelione, G T]]
[[Category: Lee H]]
[[Category: Structural genomic]]
[[Category: Montelione GT]]
[[Category: Rossi, P]]
[[Category: Rossi P]]
[[Category: Wang, H]]
[[Category: Wang H]]
[[Category: Xiao, R]]
[[Category: Xiao R]]
[[Category: Nesg]]
[[Category: PSI, Protein structure initiative]]
[[Category: Psi-biology]]
[[Category: Unknown function]]

Latest revision as of 09:56, 1 May 2024

Solution NMR Structure of the uncharacterized protein from gene locus VNG_0733H of Halobacterium salinarium, Northeast Structural Genomics Consortium Target HsR50Solution NMR Structure of the uncharacterized protein from gene locus VNG_0733H of Halobacterium salinarium, Northeast Structural Genomics Consortium Target HsR50

Structural highlights

2lok is a 1 chain structure with sequence from Halobacterium salinarum NRC-1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9HRE7_HALSA

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