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==Structure of the DNA complex of the C-Terminal domain of Ler==
==Structure of the DNA complex of the C-Terminal domain of Ler==
<StructureSection load='2lev' size='340' side='right' caption='[[2lev]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2lev' size='340' side='right'caption='[[2lev]]' scene=''>
== Structural highlights ==
== Structural highlights ==
[[2lev]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEV OCA]. <br>
<table><tr><td colspan='2'>[[2lev]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEV FirstGlance]. <br>
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solution NMR , X-ray solution scattering</td></tr>
<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lev RCSB], [http://www.ebi.ac.uk/pdbsum/2lev PDBsum]</span><br>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lev OCA], [https://pdbe.org/2lev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lev RCSB], [https://www.ebi.ac.uk/pdbsum/2lev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lev ProSAT]</span></td></tr>
== Publication Abstract from PubMed ==
</table>
Ler, a member of the H-NS protein family, is the master regulator of the LEE pathogenicity island in virulent Escherichia coli strains. Here, we determined the structure of a complex between the DNA-binding domain of Ler (CT-Ler) and a 15-mer DNA duplex. CT-Ler recognizes a preexisting structural pattern in the DNA minor groove formed by two consecutive regions which are narrower and wider, respectively, compared with standard B-DNA. The compressed region, associated with an AT-tract, is sensed by the side chain of Arg90, whose mutation abolishes the capacity of Ler to bind DNA. The expanded groove allows the approach of the loop in which Arg90 is located. This is the first report of an experimental structure of a DNA complex that includes a protein belonging to the H-NS family. The indirect readout mechanism not only explains the capacity of H-NS and other H-NS family members to modulate the expression of a large number of genes but also the origin of the specificity displayed by Ler. Our results point to a general mechanism by which horizontally acquired genes may be specifically recognized by members of the H-NS family.
== Function ==
 
[https://www.uniprot.org/uniprot/Q7DB51_ECO57 Q7DB51_ECO57]
Indirect DNA Readout by an H-NS Related Protein: Structure of the DNA Complex of the C-Terminal Domain of Ler.,Cordeiro TN, Schmidt H, Madrid C, Juarez A, Bernado P, Griesinger C, Garcia J, Pons M PLoS Pathog. 2011 Nov;7(11):e1002380. Epub 2011 Nov 17. PMID:22114557<ref>PMID:22114557</ref>
 
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Bernado, P.]]
[[Category: Large Structures]]
[[Category: Cordeiro, T N.]]
[[Category: Bernado P]]
[[Category: Garcia, J.]]
[[Category: Cordeiro TN]]
[[Category: Grisienger, C.]]
[[Category: Garcia J]]
[[Category: Juarez, A.]]
[[Category: Grisienger C]]
[[Category: Madrid, C.]]
[[Category: Juarez A]]
[[Category: Pons, M.]]
[[Category: Madrid C]]
[[Category: Schimdt, H.]]
[[Category: Pons M]]
[[Category: Arginine-minor-groove recognition]]
[[Category: Schimdt H]]
[[Category: Horizontal gene transfer]]
[[Category: Indirect readout]]
[[Category: Nucleoid associated protein]]
[[Category: Transcription regulator-dna complex]]

Latest revision as of 09:55, 1 May 2024

Structure of the DNA complex of the C-Terminal domain of LerStructure of the DNA complex of the C-Terminal domain of Ler

Structural highlights

2lev is a 3 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Hybrid , Solution NMR , X-ray solution scattering
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q7DB51_ECO57

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