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==Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant==
==Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant==
<StructureSection load='2lc9' size='340' side='right' caption='[[2lc9]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='2lc9' size='340' side='right'caption='[[2lc9]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2lc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LC9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2lc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LC9 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dmv|3dmv]], [[3dmx|3dmx]], [[2lcb|2lcb]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lc9 OCA], [https://pdbe.org/2lc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lc9 RCSB], [https://www.ebi.ac.uk/pdbsum/2lc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lc9 ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
</table>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lc9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lc9 RCSB], [http://www.ebi.ac.uk/pdbsum/2lc9 PDBsum]</span></td></tr>
== Function ==
<table>
[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Proteins are inherently plastic molecules, whose function often critically depends on excursions between different molecular conformations (conformers). However, a rigorous understanding of the relation between a protein's structure, dynamics and function remains elusive. This is because many of the conformers on its energy landscape are only transiently formed and marginally populated (less than a few per cent of the total number of molecules), so that they cannot be individually characterized by most biophysical tools. Here we study a lysozyme mutant from phage T4 that binds hydrophobic molecules and populates an excited state transiently (about 1 ms) to about 3% at 25 degrees C (ref. 5). We show that such binding occurs only via the ground state, and present the atomic-level model of the 'invisible', excited state obtained using a combined strategy of relaxation-dispersion NMR (ref. 6) and CS-Rosetta model building that rationalizes this observation. The model was tested using structure-based design calculations identifying point mutants predicted to stabilize the excited state relative to the ground state. In this way a pair of mutations were introduced, inverting the relative populations of the ground and excited states and altering function. Our results suggest a mechanism for the evolution of a protein's function by changing the delicate balance between the states on its energy landscape. More generally, they show that our approach can generate and validate models of excited protein states.
 
Solution structure of a minor and transiently formed state of a T4 lysozyme mutant.,Bouvignies G, Vallurupalli P, Hansen DF, Correia BE, Lange O, Bah A, Vernon RM, Dahlquist FW, Baker D, Kay LE Nature. 2011 Aug 21;477(7362):111-4. doi: 10.1038/nature10349. PMID:21857680<ref>PMID:21857680</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Enterobacteria phage t4]]
[[Category: Escherichia virus T4]]
[[Category: Lysozyme]]
[[Category: Large Structures]]
[[Category: Bah, A.]]
[[Category: Bah A]]
[[Category: Baker, D.]]
[[Category: Baker D]]
[[Category: Bouvignies, G.]]
[[Category: Bouvignies G]]
[[Category: Correia, B.]]
[[Category: Correia B]]
[[Category: Dahlquist, F W.]]
[[Category: Dahlquist FW]]
[[Category: Hansen, D.]]
[[Category: Hansen D]]
[[Category: Kay, L E.]]
[[Category: Kay LE]]
[[Category: Lange, O.]]
[[Category: Lange O]]
[[Category: Vallurupalli, P.]]
[[Category: Vallurupalli P]]
[[Category: Vernon, R M.]]
[[Category: Vernon RM]]
[[Category: Hydrolase]]

Latest revision as of 09:55, 1 May 2024

Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme MutantSolution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant

Structural highlights

2lc9 is a 1 chain structure with sequence from Escherichia virus T4. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENLYS_BPT4 Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.[1]

See Also

References

  1. ↑ Moussa SH, Kuznetsov V, Tran TA, Sacchettini JC, Young R. Protein determinants of phage T4 lysis inhibition. Protein Sci. 2012 Apr;21(4):571-82. doi: 10.1002/pro.2042. Epub 2012 Mar 2. PMID:22389108 doi:http://dx.doi.org/10.1002/pro.2042
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