2kx9: Difference between revisions

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[[Image:2kx9.png|left|200px]]


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==Solution Structure of the Enzyme I dimer Using Residual Dipolar Couplings and Small Angle X-Ray Scattering==
The line below this paragraph, containing "STRUCTURE_2kx9", creates the "Structure Box" on the page.
<StructureSection load='2kx9' size='340' side='right'caption='[[2kx9]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2kx9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KX9 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solution NMR , X-ray solution scattering</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kx9 OCA], [https://pdbe.org/2kx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kx9 RCSB], [https://www.ebi.ac.uk/pdbsum/2kx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kx9 ProSAT]</span></td></tr>
{{STRUCTURE_2kx9| PDB=2kx9 |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/PT1_ECOLI PT1_ECOLI] General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).<ref>PMID:7876255</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/2kx9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kx9 ConSurf].
<div style="clear:both"></div>


===Solution Structure of the Enzyme I dimer Using Residual Dipolar Couplings and Small Angle X-Ray Scattering===
==See Also==
 
*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
 
== References ==
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<references/>
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[[Category: Escherichia coli K-12]]
{{ABSTRACT_PUBMED_20731394}}
[[Category: Large Structures]]
 
[[Category: Clore G]]
==About this Structure==
[[Category: Grishaev A]]
2KX9 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KX9 OCA].
[[Category: Guirlando R]]
 
[[Category: Schwieters CD]]
==Reference==
[[Category: Suh J]]
<ref group="xtra">PMID:20731394</ref><references group="xtra"/>
[[Category: Takayama Y]]
[[Category: Escherichia coli]]
[[Category: Phosphoenolpyruvate--protein phosphotransferase]]
[[Category: Clore, G.]]
[[Category: Grishaev, A.]]
[[Category: Guirlando, R.]]
[[Category: Schwieters, C D.]]
[[Category: Suh, J.]]
[[Category: Takayama, Y.]]
[[Category: Pt]]
[[Category: Sugar phosphotransferase system]]
[[Category: Transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 13 10:15:02 2010''

Latest revision as of 09:49, 1 May 2024

Solution Structure of the Enzyme I dimer Using Residual Dipolar Couplings and Small Angle X-Ray ScatteringSolution Structure of the Enzyme I dimer Using Residual Dipolar Couplings and Small Angle X-Ray Scattering

Structural highlights

2kx9 is a 2 chain structure with sequence from Escherichia coli K-12. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Hybrid , Solution NMR , X-ray solution scattering
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PT1_ECOLI General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Powell BS, Court DL, Inada T, Nakamura Y, Michotey V, Cui X, Reizer A, Saier MH Jr, Reizer J. Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant. J Biol Chem. 1995 Mar 3;270(9):4822-39. PMID:7876255
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