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==F98N apoflavodoxin from Anabaena PCC 7119==
==F98N apoflavodoxin from Anabaena PCC 7119==
<StructureSection load='2kqu' size='340' side='right' caption='[[2kqu]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2kqu' size='340' side='right'caption='[[2kqu]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2kqu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_pcc7119 Anabaena pcc7119]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KQU FirstGlance]. <br>
<table><tr><td colspan='2'>[[2kqu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7119 Nostoc sp. PCC 7119]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KQU FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ftg|1ftg]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kqu OCA], [http://pdbe.org/2kqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kqu RCSB], [http://www.ebi.ac.uk/pdbsum/2kqu PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kqu OCA], [https://pdbe.org/2kqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kqu RCSB], [https://www.ebi.ac.uk/pdbsum/2kqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kqu ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/FLAV_NOSSO FLAV_NOSSO]] Low-potential electron donor to a number of redox enzymes.  
[https://www.uniprot.org/uniprot/FLAV_NOSSO FLAV_NOSSO] Low-potential electron donor to a number of redox enzymes.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kq/2kqu_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kq/2kqu_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kqu ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Partly unfolded protein conformations close to the native state may play important roles in protein function and in protein misfolding. Structural analyses of such conformations which are essential for their fully physicochemical understanding are complicated by their characteristic low populations at equilibrium. We stabilize here with a single mutation the equilibrium intermediate of apoflavodoxin thermal unfolding and determine its solution structure by NMR. It consists of a large native region identical with that observed in the X-ray structure of the wild-type protein plus an unfolded region. Small-angle X-ray scattering analysis indicates that the calculated ensemble of structures is consistent with the actual degree of expansion of the intermediate. The unfolded region encompasses discontinuous sequence segments that cluster in the 3D structure of the native protein forming the FMN cofactor binding loops and the binding site of a variety of partner proteins. Analysis of the apoflavodoxin inner interfaces reveals that those becoming destabilized in the intermediate are more polar than other inner interfaces of the protein. Natively folded proteins contain hydrophobic cores formed by the packing of hydrophobic surfaces, while natively unfolded proteins are rich in polar residues. The structure of the apoflavodoxin thermal intermediate suggests that the regions of natively folded proteins that are easily responsive to thermal activation may contain cores of intermediate hydrophobicity.
Design and structure of an equilibrium protein folding intermediate: a hint into dynamical regions of proteins.,Ayuso-Tejedor S, Angarica VE, Bueno M, Campos LA, Abian O, Bernado P, Sancho J, Jimenez MA J Mol Biol. 2010 Jul 23;400(4):922-34. Epub 2010 May 27. PMID:20553732<ref>PMID:20553732</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2kqu" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Flavodoxin|Flavodoxin]]
*[[Flavodoxin 3D structures|Flavodoxin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Anabaena pcc7119]]
[[Category: Large Structures]]
[[Category: Ayuso-Tejedor, S]]
[[Category: Nostoc sp. PCC 7119]]
[[Category: Bernado, P]]
[[Category: Ayuso-Tejedor S]]
[[Category: Jimenez, M A]]
[[Category: Bernado P]]
[[Category: Sancho, J]]
[[Category: Jimenez MA]]
[[Category: Electron transport]]
[[Category: Sancho J]]
[[Category: Flavoprotein]]
[[Category: Fmn]]
[[Category: Folding intermediate]]
[[Category: Transport]]

Latest revision as of 09:47, 1 May 2024

F98N apoflavodoxin from Anabaena PCC 7119F98N apoflavodoxin from Anabaena PCC 7119

Structural highlights

2kqu is a 1 chain structure with sequence from Nostoc sp. PCC 7119. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FLAV_NOSSO Low-potential electron donor to a number of redox enzymes.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

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OCA
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