2kfe: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:


==Solution structure of meucin-24==
==Solution structure of meucin-24==
<StructureSection load='2kfe' size='340' side='right'caption='[[2kfe]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2kfe' size='340' side='right'caption='[[2kfe]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2kfe]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFE FirstGlance]. <br>
<table><tr><td colspan='2'>[[2kfe]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFE FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ojm|2ojm]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfe OCA], [https://pdbe.org/2kfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfe RCSB], [https://www.ebi.ac.uk/pdbsum/2kfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfe ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfe OCA], [https://pdbe.org/2kfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfe RCSB], [https://www.ebi.ac.uk/pdbsum/2kfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfe ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plasmodium falciparum is a pathogen of human malaria which causes millions of deaths per year due to the ever-increasing drug resistance by the parasite, and thus novel antimalarial agents are urgently needed. In this work, we report two cDNA clones from the scorpion Mesobuthus eupeus venom gland, which encode peptides inhibiting the development of Plasmodium berghei, killing intraerythrocytic P. falciparum, and toxic to the Drosophila S2 cell at micromolar concentrations. One peptide of 24 amino acids (named meucin-24) shares high sequence identity to the amino-terminus of a family of scorpion venom long-chain K(+) channel toxins (LcKTx) and two frog antimicrobial peptides (magainin1 and 2). Sequencing genomic DNA of meucin-24 identified this short peptide as a product of a putative guanine-to-adenine RNA editing from a M. eupeus LcKTx transcript. Another peptide, named meucin-25, contains 25 residues and does not share sequence similarity with any known peptides. Circular dichroism analysis of chemically synthesized peptides demonstrates that meucin-24 presents an essential random coil conformation in water, but its alpha-helical content largely increases in the presence of 50% trifluoroethanol, a membrane-mimicking environment. This finding was further verified by its NMR structure that showed an alpha-helical amphipathic architecture in the region of residues 4-20. CD results indicate that meucin-25 mainly adopts a beta-sheet structure in water but TFE promotes its alpha-helical formation, suggesting its conformational flexibility. Killing of intraerythrocytic P. falciparum without harming mammalian cells (erythrocytes and GC-2 cell) make them attractive candidates for antimalarial drug design.
Characterization of two linear cationic antimalarial peptides in the scorpion Mesobuthus eupeus.,Gao B, Xu J, Rodriguez Mdel C, Lanz-Mendoza H, Hernandez-Rivas R, Du W, Zhu S Biochimie. 2010 Apr;92(4):350-9. Epub 2010 Jan 22. PMID:20097251<ref>PMID:20097251</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2kfe" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]]
*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Du, W]]
[[Category: Du W]]
[[Category: Gao, B]]
[[Category: Gao B]]
[[Category: Xu, J]]
[[Category: Xu J]]
[[Category: Zhu, S]]
[[Category: Zhu S]]
[[Category: Alpha-helix]]
[[Category: Antimicrobial protein]]

Latest revision as of 09:45, 1 May 2024

Solution structure of meucin-24Solution structure of meucin-24

Structural highlights

2kfe is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=2kfe&oldid=4135791"