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{{Large structure}}
 
==Solution structure and dynamics of S100A5 in the apo and Ca2+ -bound states==
==Solution structure and dynamics of S100A5 in the apo and Ca2+ -bound states==
<StructureSection load='2kax' size='340' side='right' caption='[[2kax]], [[NMR_Ensembles_of_Models | 31 NMR models]]' scene=''>
<StructureSection load='2kax' size='340' side='right'caption='[[2kax]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2kax]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KAX FirstGlance]. <br>
<table><tr><td colspan='2'>[[2kax]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KAX FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kay|2kay]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kax OCA], [http://pdbe.org/2kax PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kax RCSB], [http://www.ebi.ac.uk/pdbsum/2kax PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kax ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kax OCA], [https://pdbe.org/2kax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kax RCSB], [https://www.ebi.ac.uk/pdbsum/2kax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kax ProSAT]</span></td></tr>
</table>
</table>
{{Large structure}}
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/S10A5_HUMAN S10A5_HUMAN]] Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.<ref>PMID:10882717</ref>
[https://www.uniprot.org/uniprot/S10A5_HUMAN S10A5_HUMAN] Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.<ref>PMID:10882717</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kax ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kax ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
S100A5 is a calcium binding protein of the S100 family, with one canonical and one S100-specific EF-hand motif per subunit. Although its function is still unknown, it has recently been reported to be one of the S100 proteins able to interact with the receptor for advanced glycation end products. The homodimeric solution structures of S100A5 in both the apo and the calcium(II)-loaded forms have been obtained, and show a conformational rearrangement upon calcium binding. This rearrangement involves, in particular, the hinge loop connecting the N-terminal and the C-terminal EF-hand domains, the reorientation of helix III with respect to helix IV, as common to several S100 proteins, and the elongation of helix IV. The details of the structural changes are important because they must be related to the different functions, still largely unknown, of the different members of the S100 family. For the first time for a full-length S100 protein, relaxation measurements were performed on both the apo and the calcium-bound forms. A quite large mobility was observed in the hinge loop, which is not quenched in the calcium form. The structural differences resulting upon calcium binding change the global shape and the distribution of hydrophobic and charged residues of the S100A5 homodimer in a modest but significantly different manner with respect to the closest homologues S100A4 and S100A6.
Solution structure and dynamics of S100A5 in the apo and Ca(2+)-bound states.,Bertini I, Das Gupta S, Hu X, Karavelas T, Luchinat C, Parigi G, Yuan J J Biol Inorg Chem. 2009 Jun 18. PMID:19536568<ref>PMID:19536568</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2kax" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[S100 protein|S100 protein]]
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Bertini, I]]
[[Category: Large Structures]]
[[Category: Gupta, S Das]]
[[Category: Bertini I]]
[[Category: Hu, X]]
[[Category: Das Gupta S]]
[[Category: Karavelas, T]]
[[Category: Hu X]]
[[Category: Luchinat, C]]
[[Category: Karavelas T]]
[[Category: Parigi, G]]
[[Category: Luchinat C]]
[[Category: SPINE, Structural Proteomics in Europe]]
[[Category: Parigi G]]
[[Category: Yuan, J]]
[[Category: Yuan J]]
[[Category: Calcium]]
[[Category: Calcium binding protien]]
[[Category: Ef-hand]]
[[Category: Metal binding protein]]
[[Category: Polymorphism]]
[[Category: S100a5]]
[[Category: Spine]]
[[Category: Spine2]]
[[Category: Structural genomic]]
[[Category: Structural proteomics in europe]]

Latest revision as of 09:44, 1 May 2024

Solution structure and dynamics of S100A5 in the apo and Ca2+ -bound statesSolution structure and dynamics of S100A5 in the apo and Ca2+ -bound states

Structural highlights

2kax is a 2 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

S10A5_HUMAN Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Schafer BW, Fritschy JM, Murmann P, Troxler H, Durussel I, Heizmann CW, Cox JA. Brain S100A5 is a novel calcium-, zinc-, and copper ion-binding protein of the EF-hand superfamily. J Biol Chem. 2000 Sep 29;275(39):30623-30. PMID:10882717 doi:http://dx.doi.org/10.1074/jbc.M002260200
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