2j55: Difference between revisions

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-[[Image:2j55.gif|left|200px]]<br /><applet load="2j55" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2j55, resolution 2.15&Aring;" />
-'''X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE O-QUINONE IN COMPLEX WITH AMICYANIN.'''<br />
-==Overview==
+==X-ray reduced Paraccocus denitrificans methylamine dehydrogenase O- quinone in complex with amicyanin.==
-X-ray exposure during crystallographic data collection can result in, unintended redox changes in proteins containing functionally important, redox centers. In order to directly monitor X-ray-derived redox changes in, trapped oxidative half-reaction intermediates of Paracoccus denitrificans, methylamine dehydrogenase, a commercially available single-crystal UV/Vis, microspectrophotometer was installed on-line at the BioCARS beamline, 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox, state of the intermediates during X-ray exposure permitted the creation of, a general multi-crystal data collection strategy to generate true, structures of each redox intermediate.
+<StructureSection load='2j55' size='340' side='right'caption='[[2j55]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2j55]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J55 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j55 OCA], [https://pdbe.org/2j55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j55 RCSB], [https://www.ebi.ac.uk/pdbsum/2j55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j55 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AMCY_PARDE AMCY_PARDE] Primary acceptor of electrons from methylamine dehydrogenase. Passes those electrons on either a soluble cytochrome c or to pseudoazurin.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j5/2j55_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j55 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-J55 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans] with CU and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amine_dehydrogenase Amine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3] Known structural/functional Site: <scene name='pdbsite=AC1:Gol Binding Site For Chain L'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J55 OCA].
+*[[Amicyanin 3D structures|Amicyanin 3D structures]]
+*[[Methylamine dehydrogenase|Methylamine dehydrogenase]]
-==Reference==
+__TOC__
-Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry., Pearson AR, Pahl R, Kovaleva EG, Davidson VL, Wilmot CM, J Synchrotron Radiat. 2007 Jan;14(Pt 1):92-8. Epub 2006 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17211075 17211075]
+</StructureSection>
-[[Category: Amine dehydrogenase]]
+[[Category: Large Structures]]
 [[Category: Paracoccus denitrificans]]
-[[Category: Protein complex]]
+[[Category: Davidson VL]]
-[[Category: Davidson, V.]]
+[[Category: Pahl R]]
-[[Category: Pahl, R.]]
+[[Category: Pearson AR]]
-[[Category: Pearson, A.R.]]
+[[Category: Wilmot CM]]
-[[Category: Wilmot, C.M.]]
-[[Category: CU]]
-[[Category: GOL]]
-[[Category: copper]]
-[[Category: electron transport]]
-[[Category: metal-binding]]
-[[Category: methylamine dehydrogenase single crystal microspectrophoto]]
-[[Category: oxidoreductase]]
-[[Category: periplasmic]]
-[[Category: transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:49:15 2007''