2gqc: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2gqc.jpg|left|200px]]<br /><applet load="2gqc" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2gqc" />
'''Solution structure of the N-terminal domain of Rhomboid Intramembrane Protease from P. aeruginosa'''<br />


==Overview==
==Solution structure of the N-terminal domain of Rhomboid Intramembrane Protease from P. aeruginosa==
Rhomboids are ubiquitous integral membrane proteases that release cellular signals from membrane-bound substrates through a general signal transduction mechanism known as regulated intramembrane proteolysis (RIP). We present the NMR structure of the cytosolic N-terminal domain (NRho) of P. aeruginosa Rhomboid. NRho consists of a novel alpha/beta fold and represents the first detailed structural insight into this class of intramembrane proteases. We find evidence that NRho is capable of strong and specific association with detergent micelles that mimic the membrane/water interface. Relaxation measurements on NRho reveal structural fluctuations on the microseconds-milliseconds timescale in regions including and contiguous to those implicated in membrane interaction. This structural plasticity may facilitate the ability of NRho to recognize and associate with membranes. We suggest that NRho plays a role in scissile peptide bond selectivity by optimally positioning the Rhomboid active site relative to the membrane plane.
<StructureSection load='2gqc' size='340' side='right'caption='[[2gqc]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2gqc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GQC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gqc OCA], [https://pdbe.org/2gqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gqc RCSB], [https://www.ebi.ac.uk/pdbsum/2gqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gqc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HZC2_PSEAE Q9HZC2_PSEAE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gq/2gqc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gqc ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2GQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Active as [http://en.wikipedia.org/wiki/Rhomboid_protease Rhomboid protease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.105 3.4.21.105] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQC OCA].
*[[Rhomboid protease|Rhomboid protease]]
 
__TOC__
==Reference==
</StructureSection>
Solution structure and dynamics of the N-terminal cytosolic domain of rhomboid intramembrane protease from Pseudomonas aeruginosa: insights into a functional role in intramembrane proteolysis., Del Rio A, Dutta K, Chavez J, Ubarretxena-Belandia I, Ghose R, J Mol Biol. 2007 Jan 5;365(1):109-22. Epub 2006 Sep 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17059825 17059825]
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa pao1]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Rhomboid protease]]
[[Category: Chavez J]]
[[Category: Single protein]]
[[Category: Del Rio A]]
[[Category: Chavez, J.]]
[[Category: Dutta K]]
[[Category: Dutta, K.]]
[[Category: Ghose R]]
[[Category: Ghose, R.]]
[[Category: Ubarretxena-Belandia I]]
[[Category: Rio, A Del.]]
[[Category: Ubarretxena-Belandia, I.]]
[[Category: alpha-beta domain]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:34:13 2008''

Latest revision as of 09:40, 1 May 2024

Solution structure of the N-terminal domain of Rhomboid Intramembrane Protease from P. aeruginosaSolution structure of the N-terminal domain of Rhomboid Intramembrane Protease from P. aeruginosa

Structural highlights

2gqc is a 1 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9HZC2_PSEAE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=2gqc&oldid=4135505"