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| [[Image:2f8u.gif|left|200px]]
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| {{Structure
| | ==G-quadruplex structure formed in human Bcl-2 promoter, hybrid form== |
| |PDB= 2f8u |SIZE=350|CAPTION= <scene name='initialview01'>2f8u</scene>
| | <StructureSection load='2f8u' size='340' side='right'caption='[[2f8u]]' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| | <table><tr><td colspan='2'>[[2f8u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8U FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8u OCA], [https://pdbe.org/2f8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8u RCSB], [https://www.ebi.ac.uk/pdbsum/2f8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8u ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | __TOC__ |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8u OCA], [http://www.ebi.ac.uk/pdbsum/2f8u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f8u RCSB]</span>
| | </StructureSection> |
| }}
| | [[Category: Large Structures]] |
| | | [[Category: Carver M]] |
| '''Solution structure of G-quadruplex formed in the human Bcl-2 promoter'''
| | [[Category: Chen D]] |
| | | [[Category: Dai J]] |
| | | [[Category: Yang D]] |
| ==Overview==
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| BCL2 protein functions as an inhibitor of cell apoptosis and has been found to be aberrantly expressed in a wide range of human diseases. A highly GC-rich region upstream of the P1 promoter plays an important role in the transcriptional regulation of BCL2. Here we report the NMR solution structure of the major intramolecular G-quadruplex formed on the G-rich strand of this region in K+ solution. This well-defined mixed parallel/antiparallel-stranded G-quadruplex structure contains three G-tetrads of mixed G-arrangements, which are connected with two lateral loops and one side loop, and four grooves of different widths. The three loops interact with the core G-tetrads in a specific way that defines and stabilizes the overall G-quadruplex structure. The loop conformations are in accord with the experimental mutation and footprinting data. The first 3-nt loop adopts a lateral loop conformation and appears to determine the overall folding of the BCL2 G-quadruplex. The third 1-nt double-chain-reversal loop defines another example of a stable parallel-stranded structural motif using the G3NG3 sequence. Significantly, the distinct major BCL2 promoter G-quadruplex structure suggests that it can be specifically involved in gene modulation and can be an attractive target for pathway-specific drug design.
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| ==About this Structure==
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| 2F8U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8U OCA].
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| ==Reference==
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| NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region., Dai J, Chen D, Jones RA, Hurley LH, Yang D, Nucleic Acids Res. 2006;34(18):5133-44. Epub 2006 Sep 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16998187 16998187]
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| [[Category: Protein complex]] | |
| [[Category: Carver, M.]] | |
| [[Category: Chen, D.]] | |
| [[Category: Dai, J.]] | |
| [[Category: Yang, D.]] | |
| [[Category: bcl-2 promoter]]
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| [[Category: dna]]
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| [[Category: g-quadruplex]]
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| [[Category: nmr]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:59:31 2008''
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