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| [[Image:2f8u.gif|left|200px]]<br /><applet load="2f8u" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2f8u" />
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| '''Solution structure of G-quadruplex formed in the human Bcl-2 promoter'''<br />
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| ==Overview== | | ==G-quadruplex structure formed in human Bcl-2 promoter, hybrid form== |
| BCL2 protein functions as an inhibitor of cell apoptosis and has been found to be aberrantly expressed in a wide range of human diseases. A highly GC-rich region upstream of the P1 promoter plays an important role in the transcriptional regulation of BCL2. Here we report the NMR solution structure of the major intramolecular G-quadruplex formed on the G-rich strand of this region in K+ solution. This well-defined mixed parallel/antiparallel-stranded G-quadruplex structure contains three G-tetrads of mixed G-arrangements, which are connected with two lateral loops and one side loop, and four grooves of different widths. The three loops interact with the core G-tetrads in a specific way that defines and stabilizes the overall G-quadruplex structure. The loop conformations are in accord with the experimental mutation and footprinting data. The first 3-nt loop adopts a lateral loop conformation and appears to determine the overall folding of the BCL2 G-quadruplex. The third 1-nt double-chain-reversal loop defines another example of a stable parallel-stranded structural motif using the G3NG3 sequence. Significantly, the distinct major BCL2 promoter G-quadruplex structure suggests that it can be specifically involved in gene modulation and can be an attractive target for pathway-specific drug design.
| | <StructureSection load='2f8u' size='340' side='right'caption='[[2f8u]]' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[2f8u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8U FirstGlance]. <br> |
| 2F8U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8U OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8u OCA], [https://pdbe.org/2f8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8u RCSB], [https://www.ebi.ac.uk/pdbsum/2f8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8u ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region., Dai J, Chen D, Jones RA, Hurley LH, Yang D, Nucleic Acids Res. 2006;34(18):5133-44. Epub 2006 Sep 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16998187 16998187]
| | __TOC__ |
| [[Category: Protein complex]]
| | </StructureSection> |
| [[Category: Carver, M.]] | | [[Category: Large Structures]] |
| [[Category: Chen, D.]] | | [[Category: Carver M]] |
| [[Category: Dai, J.]] | | [[Category: Chen D]] |
| [[Category: Yang, D.]] | | [[Category: Dai J]] |
| [[Category: bcl-2 promoter]] | | [[Category: Yang D]] |
| [[Category: dna]] | |
| [[Category: g-quadruplex]] | |
| [[Category: nmr]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:53 2008''
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