2erm: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2erm.gif|left|200px]]<br />
-<applet load="2erm" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2erm" />
-'''Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue'''<br />
-==Overview==
+==Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue==
-The 3D structure of a complex formed by the acidic fibroblast growth, factor (FGF-1) and a specifically designed synthetic heparin, hexasaccharide has been determined by NMR spectroscopy. This, hexasaccharide can substitute natural heparins in FGF-1 mitogenesis, assays, in spite of not inducing any apparent dimerization of the growth, factor. The use of this well defined synthetic heparin analogue has, allowed us to perform a detailed NMR structural analysis of the, heparin-FGF interaction, overcoming the limitations of NMR to deal with, the high molecular mass and heterogeneity of the FGF-1 oligomers formed in, the presence of natural heparin fragments. Our results confirm that, glycosaminoglycans induced FGF-1 dimerization either in a cis or trans, disposition with respect to the heparin chain is not an absolute, requirement for biological activity.
+<StructureSection load='2erm' size='340' side='right'caption='[[2erm]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2erm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ERM FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNS:N-SULFO-ALPHA-D-GLUCOSAMINE'>GNS</scene>, <scene name='pdbligand=IDR:L-IDURONIC+ACID'>IDR</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NGY:2-(ACETYLAMINO)-2-DEOXY-6-O-SULFO-ALPHA-D-GLUCOPYRANOSE'>NGY</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2erm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2erm OCA], [https://pdbe.org/2erm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2erm RCSB], [https://www.ebi.ac.uk/pdbsum/2erm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2erm ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FGF1_HUMAN FGF1_HUMAN] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:8663044</ref> <ref>PMID:16597617</ref> <ref>PMID:20145243</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/2erm_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2erm ConSurf].
+<div style="clear:both"></div>
-==Disease==
+==See Also==
-Known diseases associated with this structure: Aplasia of lacrimal and salivary glands OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=602115 602115]], LADD syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=602115 602115]]
+*[[Fibroblast growth factor 3D structures|Fibroblast growth factor 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-ERM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with IPA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ERM OCA].
+__TOC__
+</StructureSection>
-==Reference==
-Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue., Canales A, Lozano R, Lopez-Mendez B, Angulo J, Ojeda R, Nieto PM, Martin-Lomas M, Gimenez-Gallego G, Jimenez-Barbero J, FEBS J. 2006 Oct;273(20):4716-27. Epub 2006 Sep 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16995857 16995857]
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Canales, A.]]
+[[Category: Canales A]]
-[[Category: Gimenez-Gallego, G.]]
+[[Category: Gimenez-Gallego G]]
-[[Category: Jimenez-Barbero, J.]]
+[[Category: Jimenez-Barbero J]]
-[[Category: Lozano, R.]]
+[[Category: Lozano R]]
-[[Category: Martin-Lomas, M.]]
+[[Category: Martin-Lomas M]]
-[[Category: Nieto, P.M.]]
+[[Category: Nieto PM]]
-[[Category: IPA]]
-[[Category: fibroblast growth factor]]
-[[Category: heparin-like hexasaccharide]]
-[[Category: protein-carbohydrate complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:54:39 2007''