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==THIOREDOXIN (REDUCED DITHIO FORM), NMR, 20 STRUCTURES==
==THIOREDOXIN (REDUCED DITHIO FORM), NMR, 20 STRUCTURES==
<StructureSection load='1xob' size='340' side='right' caption='[[1xob]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1xob' size='340' side='right'caption='[[1xob]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1xob]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1trx 1trx]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XOB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XOB FirstGlance]. <br>
<table><tr><td colspan='2'>[[1xob]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1trx 1trx]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XOB FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xob OCA], [http://pdbe.org/1xob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xob RCSB], [http://www.ebi.ac.uk/pdbsum/1xob PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xob ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xob OCA], [https://pdbe.org/1xob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xob RCSB], [https://www.ebi.ac.uk/pdbsum/1xob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xob ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/THIO_ECOLI THIO_ECOLI]] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.  
[https://www.uniprot.org/uniprot/THIO_ECOLI THIO_ECOLI] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xo/1xob_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xo/1xob_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xob ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xob ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Thioredoxin participates in thiol-disulfide exchange reactions and both oxidized thioredoxin (disulfide form) and reduced thioredoxin (dithiol form) are found under physiological conditions. Previous structural studies suggested that the two forms were extremely similar, although significant functional and spectroscopic differences exist. We therefore undertook high-resolution solution structural studies of the two forms of Escherichia coli thioredoxin in order to detect subtle conformational differences. RESULTS: The solution structures of reduced and oxidized thioredoxin are extremely similar. Backbone structure is largely identical in the two forms, with slight differences in the region of the active site, which includes Cys32 and Cys35. The side chain sulfur atom of Cys32 is tilted away from that of Cys35 in the reduced form of the protein to accommodate the increase in S-S distance that occurs upon reduction of the disulfide, but the chi 1 angles of the two cysteines remain the same in the two forms. CONCLUSIONS: Only subtle conformational changes occur upon changing the oxidation state of the active site cysteines, including the positions of some side chains and in hydrogen bonding patterns in the active site region. Functional differences between the two forms are probably therefore related to differences in local conformational flexibility in and near the active site loop.


High-resolution solution structures of oxidized and reduced Escherichia coli thioredoxin.,Jeng MF, Campbell AP, Begley T, Holmgren A, Case DA, Wright PE, Dyson HJ Structure. 1994 Sep 15;2(9):853-68. PMID:7812718<ref>PMID:7812718</ref>
==See Also==
 
*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1xob" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Escherichia coli]]
[[Category: Begley, T]]
[[Category: Large Structures]]
[[Category: Campbell, A P]]
[[Category: Begley T]]
[[Category: Case, D A]]
[[Category: Campbell AP]]
[[Category: Dyson, H J]]
[[Category: Case DA]]
[[Category: Holmgren, A]]
[[Category: Dyson HJ]]
[[Category: Jeng, M F]]
[[Category: Holmgren A]]
[[Category: Wright, P E]]
[[Category: Jeng M-F]]
[[Category: Electron transport]]
[[Category: Wright PE]]
[[Category: Redox-active center]]

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