1x9l: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1x9l.gif|left|200px]]<br /><applet load="1x9l" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1x9l" />
-'''Solution structure of CuI-DR1885 from Deinococcus Radiodurans'''<br />
-==Overview==
+==Solution structure of CuI-DR1885 from Deinococcus Radiodurans==
-Sco1 and Cox17 are accessory proteins required for the correct assembly of, eukaryotic cytochrome c oxidase. At variance with Sco1, Cox17 orthologs, are found only in eukaryotes. We browsed bacterial genomes to search, proteins functionally equivalent to Cox17, and we identified a class of, proteins of unknown function displaying a conserved gene neighborhood to, bacterial Sco1 genes, all sharing a potential metal binding motif, H(M)X10MX21HXM. Two members of this group, DR1885 from Deinococcus, radiodurans and CC3502 from Caulobacter crescentus, were expressed, and, their interaction with copper was investigated. The solution structure and, extended x-ray absorption fine structure data on the former protein reveal, that the protein binds copper(I) through a histidine and three Mets in a, cupredoxin-like fold. The surface location of the copper-binding site as, well as the type of coordination are well poised for metal transfer, chemistry, suggesting that DR1885 might transfer copper, taking the role, of Cox17 in bacteria. On the basis of our results, a possible pathway for, copper delivery to the Cu(A) center in bacteria is proposed.
+<StructureSection load='1x9l' size='340' side='right'caption='[[1x9l]]' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1x9l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X9L FirstGlance]. <br>
-X9L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans_r1 Deinococcus radiodurans r1] with CU1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1X9L OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x9l OCA], [https://pdbe.org/1x9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x9l RCSB], [https://www.ebi.ac.uk/pdbsum/1x9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x9l ProSAT]</span></td></tr>
-A copper(I) protein possibly involved in the assembly of CuA center of bacterial cytochrome c oxidase., Banci L, Bertini I, Ciofi-Baffoni S, Katsari E, Katsaros N, Kubicek K, Mangani S, Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):3994-9. Epub 2005 Mar 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15753304 15753304]
+</table>
-[[Category: Deinococcus radiodurans r1]]
+== Function ==
-[[Category: Single protein]]
+[https://www.uniprot.org/uniprot/Q9RT80_DEIRA Q9RT80_DEIRA]
-[[Category: Banci, L.]]
+== Evolutionary Conservation ==
-[[Category: Bertini, I.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Ciofi-Baffoni, S.]]
+Check<jmol>
-[[Category: Katsari, E.]]
+  <jmolCheckbox>
-[[Category: Katsaros, N.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x9/1x9l_consurf.spt"</scriptWhenChecked>
-[[Category: Kubicek, K.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: SPINE, Structural.Proteomics.in.Europe.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: CU1]]
+  </jmolCheckbox>
-[[Category: copper binding protein]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x9l ConSurf].
-[[Category: deinococcus radiodurans]]
+<div style="clear:both"></div>
-[[Category: holo-form]]
+__TOC__
-[[Category: nmr]]
+</StructureSection>
-[[Category: solution structure]]
+[[Category: Deinococcus radiodurans R1]]
-[[Category: spine]]
+[[Category: Large Structures]]
-[[Category: structural genomics]]
+[[Category: Banci L]]
-[[Category: structural proteomics in europe]]
+[[Category: Bertini I]]
+[[Category: Ciofi-Baffoni S]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:43:20 2007''
+[[Category: Katsari E]]
+[[Category: Katsaros N]]
+[[Category: Kubicek K]]