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| [[Image:1x93.gif|left|200px]]<br /><applet load="1x93" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1x93" />
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| '''NMR Structure of Helicobacter pylori HP0222'''<br />
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| ==Overview== | | ==NMR Structure of Helicobacter pylori HP0222== |
| Helicobacter pylori is a widespread human bacterial pathogen responsible for inducing gastric and duodenal ulcers and gastric cancers. To date, only 16 protein structures from this organism have been determined, and more than 30% of its 1500 protein functions remain unknown. We report the biochemical characterization, the tertiary structure determined by solution nuclear magnetic resonance (NMR) methods and the putative function of the previously uncharacterized protein HP0222 (JHP0208) from H. pylori. Recombinant HP0222 behaves as a dimer in crosslinking and size exclusion chromatography experiments. The structure consists of a ribbon-helix-helix fold characteristic of transcription factors of the Arc/MetJ family, which all bind DNA as higher order oligomers. Electrophoretic mobility shift assays reveal that HP0222 binds to double-stranded DNA. Previous studies have shown significant increases in transcription levels of HP0222 in response to acid shock and adherence to gastric epithelial cells. To assess possible involvement of HP0222 in acid resistance, we constructed and assayed an H. pylori HP0222 null mutant. We propose that HP0222 is a novel transcriptional regulator in H. pylori.
| | <StructureSection load='1x93' size='340' side='right'caption='[[1x93]]' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1x93]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X93 FirstGlance]. <br> |
| 1X93 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X93 OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x93 OCA], [https://pdbe.org/1x93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x93 RCSB], [https://www.ebi.ac.uk/pdbsum/1x93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x93 ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| Helicobacter pylori protein HP0222 belongs to Arc/MetJ family of transcriptional regulators., Popescu A, Karpay A, Israel DA, Peek RM Jr, Krezel AM, Proteins. 2005 May 1;59(2):303-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15723352 15723352]
| | == Function == |
| | [https://www.uniprot.org/uniprot/O25010_HELPY O25010_HELPY] |
| | __TOC__ |
| | </StructureSection> |
| [[Category: Helicobacter pylori 26695]] | | [[Category: Helicobacter pylori 26695]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Israel, D.]] | | [[Category: Israel D]] |
| [[Category: Jr., R M.Peek.]]
| | [[Category: Karpay A]] |
| [[Category: Karpay, A.]] | | [[Category: Krezel AM]] |
| [[Category: Krezel, A M.]] | | [[Category: Peek Jr RM]] |
| [[Category: Popescu, A.]] | | [[Category: Popescu A]] |
| [[Category: arc]] | |
| [[Category: copg]]
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| [[Category: helicobacter]]
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| [[Category: hp0222]]
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| [[Category: jhp0208]]
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| [[Category: metj]]
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| [[Category: mnt]]
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| [[Category: parg]]
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| [[Category: pylori]]
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| [[Category: regulator]]
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| [[Category: repa]]
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| [[Category: transcription]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:17 2008''
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