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[[Image:1uzv.gif|left|200px]]<br />
<applet load="1uzv" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1uzv, resolution 1.00&Aring;" />
'''HIGH AFFINITY FUCOSE BINDING OF PSEUDOMONAS AERUGINOSA LECTIN II: 1.0 A CRYSTAL STRUCTURE OF THE COMPLEX'''<br />


==Overview==
==High affinity fucose binding of Pseudomonas aeruginosa lectin II: 1.0 A crystal structure of the complex==
PA-IIL is a fucose-binding lectin from Pseudomonas aeruginosa that is, closely related to the virulence factors of the bacterium. Previous, structural studies have revealed a new carbohydrate-binding mode with, direct involvement of two calcium ions (Mitchell E, Houles C, Sudakevitz, D, Wimmerova M, Gautier C, Perez S, Wu AM, Gilboa-Garber N, Imberty A., Structural basis for selective recognition of oligosaccharides from cystic, fibrosis patients by the lectin PA-IIL of Pseudomonas aeruginosa. Nat, Struct Biol 2002;9:918-921). A combination of thermodynamic, structural, and computational methods has been used to study the basis of the high, affinity for the monosaccharide ligand. A titration microcalorimetry study, indicated that the high affinity is enthalpy driven. The crystal ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15573375 (full description)]]
<StructureSection load='1uzv' size='340' side='right'caption='[[1uzv]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1uzv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UZV FirstGlance]. <br>
1UZV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with FUC, SO4 and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: C1A. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UZV OCA]].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uzv OCA], [https://pdbe.org/1uzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uzv RCSB], [https://www.ebi.ac.uk/pdbsum/1uzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uzv ProSAT]</span></td></tr>
High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches., Mitchell EP, Sabin C, Snajdrova L, Pokorna M, Perret S, Gautier C, Hofr C, Gilboa-Garber N, Koca J, Wimmerova M, Imberty A, Proteins. 2005 Feb 15;58(3):735-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15573375 15573375]
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HYN5_PSEAE Q9HYN5_PSEAE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uz/1uzv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uzv ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Budova M]]
[[Category: Budova, M.]]
[[Category: Gautier C]]
[[Category: Gautier, C.]]
[[Category: Gilboa-Garber N]]
[[Category: Gilboa-Garber, N.]]
[[Category: Imberty A]]
[[Category: Imberty, A.]]
[[Category: Koca J]]
[[Category: Koca, J.]]
[[Category: Mitchell E]]
[[Category: Mitchell, E.]]
[[Category: Perret S]]
[[Category: Perret, S.]]
[[Category: Sabin CD]]
[[Category: Sabin, C.D.]]
[[Category: Snajdrova L]]
[[Category: Snajdrova, L.]]
[[Category: Wimmerova M]]
[[Category: Wimmerova, M.]]
[[Category: CA]]
[[Category: FUC]]
[[Category: SO4]]
[[Category: calcium]]
[[Category: fucose]]
[[Category: lectin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:16:52 2007''

Latest revision as of 09:36, 1 May 2024

High affinity fucose binding of Pseudomonas aeruginosa lectin II: 1.0 A crystal structure of the complexHigh affinity fucose binding of Pseudomonas aeruginosa lectin II: 1.0 A crystal structure of the complex

Structural highlights

1uzv is a 4 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9HYN5_PSEAE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1uzv, resolution 1.00Å

Drag the structure with the mouse to rotate

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OCA
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