1sb6: Difference between revisions

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[[Image:1sb6.jpg|left|200px]]


{{Structure
==Solution structure of a cyanobacterial copper metallochaperone, ScAtx1==
|PDB= 1sb6 |SIZE=350|CAPTION= <scene name='initialview01'>1sb6</scene>
<StructureSection load='1sb6' size='340' side='right'caption='[[1sb6]]' scene=''>
|SITE=
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1sb6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SB6 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sb6 OCA], [https://pdbe.org/1sb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sb6 RCSB], [https://www.ebi.ac.uk/pdbsum/1sb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sb6 ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Solution structure of a cyanobacterial copper metallochaperone, ScAtx1'''
[https://www.uniprot.org/uniprot/P73213_SYNY3 P73213_SYNY3]  
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
The Atx1 copper metallochaperone from Synechocystis PCC 6803, ScAtx1, interacts with two P(1)-type copper ATPases to supply copper proteins within intracellular compartments, avoiding ATPases for other metals en route. Here we report NMR-derived solution structures for ScAtx1. The monomeric apo form has a betaalphabetabetaalpha fold with backbone motions largely restricted to loop 1 containing Cys-12 and Cys-15. The tumbling rate of Cu(I)ScAtx1 (0.1-0.8 mm) implies dimers. Experimental restraints are satisfied by symmetrical dimers with Cys-12 or His-61, but not Cys-15, invading the copper site of the opposing subunit. A full sequence of copper ligands from the cell surface to thylakoid compartments is proposed, considering in vitro homodimer liganding to mimic in vivo liganding in ScAtx1-ATPase heterodimers. A monomeric high resolution structure for Cu(I)ScAtx1, with Cys-12, Cys-15, and His-61 as ligands, is calculated without violations despite the rotational correlation time. (2)J(NH) couplings in the imidazole ring of His-61 establish coordination of N(epsilon2) to copper. His-61 is analogous to Lys-65 in eukaryotic metallochaperones, stabilizing Cu(I)S(2) complexes but by binding Cu(I) rather than compensating charge. Cys-Cys-His ligand sets are an emergent theme in some copper metallochaperones, although not in related Atx1, CopZ, or Hah1. Surface charge (Glu-13) close to the metal-binding site of ScAtx1 is likely to support interaction with complementary surfaces of copper-transporting ATPases (PacS-Arg-11 and CtaA-Lys-14) but to discourage interaction with zinc ATPase ZiaA and so inhibit aberrant formation of copper-ZiaA complexes.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sb/1sb6_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1SB6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SB6 OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sb6 ConSurf].
Solution structures of a cyanobacterial metallochaperone: insight into an atypical copper-binding motif., Banci L, Bertini I, Ciofi-Baffoni S, Su XC, Borrelly GP, Robinson NJ, J Biol Chem. 2004 Jun 25;279(26):27502-10. Epub 2004 Apr 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15075318 15075318]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
[[Category: Synechocystis sp.]]
</StructureSection>
[[Category: Banci, L.]]
[[Category: Large Structures]]
[[Category: Bertini, I.]]
[[Category: Synechocystis sp. PCC 6803]]
[[Category: Borrelly, G P.]]
[[Category: Banci L]]
[[Category: Ciofi-Baffoni, S.]]
[[Category: Bertini I]]
[[Category: Robinson, N J.]]
[[Category: Borrelly GP]]
[[Category: SPINE, Structural Proteomics in Europe.]]
[[Category: Ciofi-Baffoni S]]
[[Category: Su, X C.]]
[[Category: Robinson NJ]]
[[Category: copper chaperone]]
[[Category: Su XC]]
[[Category: new metal binding motif]]
[[Category: nmr]]
[[Category: spine]]
[[Category: structural genomic]]
[[Category: structural proteomics in europe]]
[[Category: structure]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:02:39 2008''

Latest revision as of 09:34, 1 May 2024

Solution structure of a cyanobacterial copper metallochaperone, ScAtx1Solution structure of a cyanobacterial copper metallochaperone, ScAtx1

Structural highlights

1sb6 is a 1 chain structure with sequence from Synechocystis sp. PCC 6803. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P73213_SYNY3

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

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