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| [[Image:1sb6.jpg|left|200px]]<br /><applet load="1sb6" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1sb6" />
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| '''Solution structure of a cyanobacterial copper metallochaperone, ScAtx1'''<br />
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| ==Overview== | | ==Solution structure of a cyanobacterial copper metallochaperone, ScAtx1== |
| The Atx1 copper metallochaperone from Synechocystis PCC 6803, ScAtx1, interacts with two P(1)-type copper ATPases to supply copper proteins within intracellular compartments, avoiding ATPases for other metals en route. Here we report NMR-derived solution structures for ScAtx1. The monomeric apo form has a betaalphabetabetaalpha fold with backbone motions largely restricted to loop 1 containing Cys-12 and Cys-15. The tumbling rate of Cu(I)ScAtx1 (0.1-0.8 mm) implies dimers. Experimental restraints are satisfied by symmetrical dimers with Cys-12 or His-61, but not Cys-15, invading the copper site of the opposing subunit. A full sequence of copper ligands from the cell surface to thylakoid compartments is proposed, considering in vitro homodimer liganding to mimic in vivo liganding in ScAtx1-ATPase heterodimers. A monomeric high resolution structure for Cu(I)ScAtx1, with Cys-12, Cys-15, and His-61 as ligands, is calculated without violations despite the rotational correlation time. (2)J(NH) couplings in the imidazole ring of His-61 establish coordination of N(epsilon2) to copper. His-61 is analogous to Lys-65 in eukaryotic metallochaperones, stabilizing Cu(I)S(2) complexes but by binding Cu(I) rather than compensating charge. Cys-Cys-His ligand sets are an emergent theme in some copper metallochaperones, although not in related Atx1, CopZ, or Hah1. Surface charge (Glu-13) close to the metal-binding site of ScAtx1 is likely to support interaction with complementary surfaces of copper-transporting ATPases (PacS-Arg-11 and CtaA-Lys-14) but to discourage interaction with zinc ATPase ZiaA and so inhibit aberrant formation of copper-ZiaA complexes.
| | <StructureSection load='1sb6' size='340' side='right'caption='[[1sb6]]' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1sb6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SB6 FirstGlance]. <br> |
| 1SB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SB6 OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sb6 OCA], [https://pdbe.org/1sb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sb6 RCSB], [https://www.ebi.ac.uk/pdbsum/1sb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sb6 ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| Solution structures of a cyanobacterial metallochaperone: insight into an atypical copper-binding motif., Banci L, Bertini I, Ciofi-Baffoni S, Su XC, Borrelly GP, Robinson NJ, J Biol Chem. 2004 Jun 25;279(26):27502-10. Epub 2004 Apr 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15075318 15075318]
| | == Function == |
| [[Category: Single protein]] | | [https://www.uniprot.org/uniprot/P73213_SYNY3 P73213_SYNY3] |
| [[Category: Synechocystis sp.]]
| | == Evolutionary Conservation == |
| [[Category: Banci, L.]]
| | [[Image:Consurf_key_small.gif|200px|right]] |
| [[Category: Bertini, I.]] | | Check<jmol> |
| [[Category: Borrelly, G P.]] | | <jmolCheckbox> |
| [[Category: Ciofi-Baffoni, S.]] | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sb/1sb6_consurf.spt"</scriptWhenChecked> |
| [[Category: Robinson, N J.]] | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| [[Category: SPINE, Structural Proteomics in Europe.]] | | <text>to colour the structure by Evolutionary Conservation</text> |
| [[Category: Su, X C.]] | | </jmolCheckbox> |
| [[Category: copper chaperone]] | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sb6 ConSurf]. |
| [[Category: new metal binding motif]] | | <div style="clear:both"></div> |
| [[Category: nmr]] | | __TOC__ |
| [[Category: spine]] | | </StructureSection> |
| [[Category: structural genomics]] | | [[Category: Large Structures]] |
| [[Category: structural proteomics in europe]] | | [[Category: Synechocystis sp. PCC 6803]] |
| [[Category: structure]] | | [[Category: Banci L]] |
| | | [[Category: Bertini I]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:43 2008''
| | [[Category: Borrelly GP]] |
| | [[Category: Ciofi-Baffoni S]] |
| | [[Category: Robinson NJ]] |
| | [[Category: Su XC]] |