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[[Image:1oko.gif|left|200px]]<br />
<applet load="1oko" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1oko, resolution 1.600&Aring;" />
'''CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN 1 COMPLEXED WITH GALACTOSE AT 1.6 A RESOLUTION'''<br />


==Overview==
==Crystal structure of Pseudomonas Aeruginosa Lectin 1 complexed with galactose at 1.6 A resolution==
The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in, complex with galactose and calcium was determined at 1.6 A resolution, and, the native protein was solved at 2.4 A resolution. Each monomer adopts a, beta-sandwich fold with ligand binding site at the apex. All galactose, hydroxyl groups, except O1, are involved in a hydrogen bond network with, the protein and O3 and O4 also participate in the co-ordination of the, calcium ion. The stereochemistry of calcium galactose binding is, reminiscent of that observed in some animal C-type lectins. The structure, of the complex provides a framework for future design of anti-bacterial, compounds.
<StructureSection load='1oko' size='340' side='right'caption='[[1oko]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1oko]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OKO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oko OCA], [https://pdbe.org/1oko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oko RCSB], [https://www.ebi.ac.uk/pdbsum/1oko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oko ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.


==About this Structure==
==See Also==
1OKO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with GAL, CA, SO4 and MPD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OKO OCA]].
*[[Galactose-binding lectin|Galactose-binding lectin]]
 
__TOC__
==Reference==
</StructureSection>
Structural basis of calcium and galactose recognition by the lectin PA-IL of Pseudomonas aeruginosa., Cioci G, Mitchell EP, Gautier C, Wimmerova M, Sudakevitz D, Perez S, Gilboa-Garber N, Imberty A, FEBS Lett. 2003 Dec 4;555(2):297-301. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14644431 14644431]
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Cioci G]]
[[Category: Cioci, G.]]
[[Category: Gautier C]]
[[Category: Gautier, C.]]
[[Category: Gilboa-Garber N]]
[[Category: Gilboa-Garber, N.]]
[[Category: Imberty A]]
[[Category: Imberty, A.]]
[[Category: Mitchell E]]
[[Category: Mitchell, E.]]
[[Category: Perez S]]
[[Category: Perez, S.]]
[[Category: Wimmerova M]]
[[Category: Wimmerova, M.]]
[[Category: CA]]
[[Category: GAL]]
[[Category: MPD]]
[[Category: SO4]]
[[Category: galactose binding]]
[[Category: lectin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:49:41 2007''

Latest revision as of 09:33, 1 May 2024

Crystal structure of Pseudomonas Aeruginosa Lectin 1 complexed with galactose at 1.6 A resolutionCrystal structure of Pseudomonas Aeruginosa Lectin 1 complexed with galactose at 1.6 A resolution

Structural highlights

1oko is a 4 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PA1L_PSEAE D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.

See Also

1oko, resolution 1.60Å

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