1n6t: Difference between revisions

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[[Image:1n6t.jpg|left|200px]]<br /><applet load="1n6t" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1n6t" />
'''Solution Structure of the Tachykinin Peptide Neurokinin A'''<br />


==Overview==
==Solution Structure of the Tachykinin Peptide Neurokinin A==
The solution structure of NKA, a decapeptide of mammalian origin, has been characterized by CD spectropolarimetry and 2D proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in both aqueous and membrane mimetic solvents. Unambiguous NMR assignments of protons have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and nuclear Overhauser effect spectroscopy (NOESY and ROESY) experiments. The distance constraints obtained from the NMR data have been utilized to generate a family of structures, which have been refined using restrained energy minimization and dynamics. These data show that in water NKA prefers to be in an extended chain conformation whereas a helical conformation is induced in the central core and the C-terminal region (D4-M10) of the peptide in the presence of perdeuterated dodecylphosphocholine (DPC) micelles, a membrane model system. Though less defined the N-terminus also displays some degree of order and a possible turn structure. The conformation adopted by NKA in the presence of DPC micelles represents a structural motif typical of neurokinin-2 selective agonists and is similar to that reported for eledoisin in hydrophobic environment.
<StructureSection load='1n6t' size='340' side='right'caption='[[1n6t]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1n6t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N6T FirstGlance]. <br>
1N6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6T OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6t OCA], [https://pdbe.org/1n6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n6t RCSB], [https://www.ebi.ac.uk/pdbsum/1n6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n6t ProSAT]</span></td></tr>
==Reference==
</table>
Three-dimensional structure of the mammalian tachykinin peptide neurokinin A bound to lipid micelles., Chandrashekar IR, Cowsik SM, Biophys J. 2003 Dec;85(6):4002-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14645089 14645089]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/TKNB_CHICK TKNB_CHICK] Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
[[Category: Chandrashekar, I R.]]
__TOC__
[[Category: Cowsik, S M.]]
</StructureSection>
[[Category: 3 10 helix]]
[[Category: Gallus gallus]]
[[Category: dpc micelles]]
[[Category: Large Structures]]
[[Category: helix]]
[[Category: Chandrashekar IR]]
[[Category: lipid induced conformation]]
[[Category: Cowsik SM]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:53 2008''

Latest revision as of 09:32, 1 May 2024

Solution Structure of the Tachykinin Peptide Neurokinin ASolution Structure of the Tachykinin Peptide Neurokinin A

Structural highlights

1n6t is a 1 chain structure with sequence from Gallus gallus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TKNB_CHICK Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

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