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| [[Image:1kks.gif|left|200px]]
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| {{Structure
| | ==Structure of the histone mRNA hairpin required for cell cycle regulation of histone gene expression== |
| |PDB= 1kks |SIZE=350|CAPTION= <scene name='initialview01'>1kks</scene>
| | <StructureSection load='1kks' size='340' side='right'caption='[[1kks]]' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| | <table><tr><td colspan='2'>[[1kks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KKS FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kks OCA], [https://pdbe.org/1kks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kks RCSB], [https://www.ebi.ac.uk/pdbsum/1kks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kks ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | __TOC__ |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kks OCA], [http://www.ebi.ac.uk/pdbsum/1kks PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kks RCSB]</span>
| | </StructureSection> |
| }}
| | [[Category: Large Structures]] |
| | | [[Category: Mus musculus]] |
| '''Structure of the histone mRNA hairpin required for cell cycle regulation of histone gene expression'''
| | [[Category: Crombie C]] |
| | | [[Category: Linge JP]] |
| | | [[Category: Luyten I]] |
| ==Overview==
| | [[Category: Muller B]] |
| Expression of replication-dependent histone genes requires a conserved hairpin RNA element in the 3' untranslated regions of poly(A)-less histone mRNAs. The 3' hairpin element is recognized by the hairpin-binding protein or stem-loop-binding protein (HBP/SLBP). This protein-RNA interaction is important for the endonucleolytic cleavage generating the mature mRNA 3' end. The 3' hairpin and presumably HBP/SLBP are also required for nucleocytoplasmic transport, translation, and stability of histone mRNAs. RNA 3' processing and mRNA stability are both regulated during the cell cycle. Here, we have determined the three-dimensional structure of a 24-mer RNA comprising a mammalian histone RNA hairpin using heteronuclear multidimensional NMR spectroscopy. The hairpin adopts a novel UUUC tetraloop conformation that is stabilized by base stacking involving the first and third loop uridines and a closing U-A base pair, and by hydrogen bonding between the first and third uridines in the tetraloop. The HBP interaction of hairpin RNA variants was analyzed in band shift experiments. Particularly important interactions for HBP recognition are mediated by the closing U-A base pair and the first and third loop uridines, whose Watson-Crick functional groups are exposed towards the major groove of the RNA hairpin. The results obtained provide novel structural insight into the interaction of the histone 3' hairpin with HBP, and thus the regulation of histone mRNA metabolism.
| | [[Category: Nilges M]] |
| | | [[Category: Sattler M]] |
| ==About this Structure==
| | [[Category: Schuemperli D]] |
| 1KKS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKS OCA].
| | [[Category: Zanier K]] |
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| ==Reference==
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| Structure of the histone mRNA hairpin required for cell cycle regulation of histone gene expression., Zanier K, Luyten I, Crombie C, Muller B, Schumperli D, Linge JP, Nilges M, Sattler M, RNA. 2002 Jan;8(1):29-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11871659 11871659]
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| [[Category: Protein complex]] | |
| [[Category: Crombie, C.]] | |
| [[Category: Linge, J P.]] | |
| [[Category: Luyten, I.]] | |
| [[Category: Muller, B]] | |
| [[Category: Nilges, M.]] | |
| [[Category: Sattler, M.]] | |
| [[Category: Schuemperli, D.]] | |
| [[Category: Zanier, K.]] | |
| [[Category: histone mrna]]
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| [[Category: rna hairpin]]
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| [[Category: rna processing]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:49:57 2008''
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