1qyp: Difference between revisions
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==THERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURES== | |||
<StructureSection load='1qyp' size='340' side='right'caption='[[1qyp]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1qyp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_celer Thermococcus celer]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QYP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
== | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyp OCA], [https://pdbe.org/1qyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qyp RCSB], [https://www.ebi.ac.uk/pdbsum/1qyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qyp ProSAT]</span></td></tr> | ||
[[1qyp]] is a 1 chain structure | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/TFS_THECE TFS_THECE] Induces RNA cleavage activity in the RNA polymerase. In its presence, the cleavage activity of the RNA polymerase truncates the RNA back to position +15 in a stepwise manner by releasing mainly dinucleotides from the 3'-end of the nascent RNA. The truncated RNAs are able to continue elongation. Involved in transcriptional proofreading and fidelity. Misincorporation of nucleotides during elongation of transcription leads to arrested elongation complexes which are rescued by TFS-promoted removal of a dinucleotide from the 3'-end. TFS is able to induce a cleavage resynthesis cycle in stalled elongation complexes (resulting from the next missing nucleotide or a reduced incorporation rate of a wrong nucleotide) preventing misincorporation and enabling proofreading in a post-incorporation manner. Pausing of elongation complexes is the main determinant of TFS-induced RNA cleavage.[UniProtKB:Q9P9I8] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qy/1qyp_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qyp ConSurf]. | |||
<div style="clear:both"></div> | |||
==See Also== | ==See Also== | ||
*[[RNA polymerase|RNA polymerase]] | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
__TOC__ | |||
</StructureSection> | |||
< | [[Category: Large Structures]] | ||
[[Category: Thermococcus celer]] | [[Category: Thermococcus celer]] | ||
[[Category: Jones | [[Category: Jones DNM]] | ||
[[Category: Kaine | [[Category: Kaine BP]] | ||
[[Category: Wang | [[Category: Wang B]] | ||
[[Category: Weiss | [[Category: Weiss MA]] | ||
Latest revision as of 09:08, 17 April 2024
THERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURESTHERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURES
Structural highlights
FunctionTFS_THECE Induces RNA cleavage activity in the RNA polymerase. In its presence, the cleavage activity of the RNA polymerase truncates the RNA back to position +15 in a stepwise manner by releasing mainly dinucleotides from the 3'-end of the nascent RNA. The truncated RNAs are able to continue elongation. Involved in transcriptional proofreading and fidelity. Misincorporation of nucleotides during elongation of transcription leads to arrested elongation complexes which are rescued by TFS-promoted removal of a dinucleotide from the 3'-end. TFS is able to induce a cleavage resynthesis cycle in stalled elongation complexes (resulting from the next missing nucleotide or a reduced incorporation rate of a wrong nucleotide) preventing misincorporation and enabling proofreading in a post-incorporation manner. Pausing of elongation complexes is the main determinant of TFS-induced RNA cleavage.[UniProtKB:Q9P9I8] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. See Also |
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